Detailed information of TA system TA209223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-AbrB
• Location: 1820363..1820927
• Replicon: chromosome
• Accession: NZ_CP079097
• Organism: Methylococcus capsulatus strain KN2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KW114_RS08255
• Protein ID: WP_218796621.1
• Coordinates: 1820583..1820927 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KW114_RS08250
• Protein ID: WP_218796620.1
• Coordinates: 1820363..1820593 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW114_RS08245 (KW114_08245)	1816647..1820321	+	3675	WP_218796619.1	helicase-related protein	-
KW114_RS08250 (KW114_08250)	1820363..1820593	+	231	WP_218796620.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
KW114_RS08255 (KW114_08255)	1820583..1820927	+	345	WP_218796621.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KW114_RS08260 (KW114_08260)	1820924..1823956	+	3033	WP_218796622.1	DUF1156 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-109)

Antitoxin

• PrlF antitoxin (5-47)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12092.96 Da
• Isoelectric Point: 8.5241

>T209223 WP_218796621.1 NZ_CP079097:1820583-1820927 [Methylococcus capsulatus]
VTYERGQIVRVPFPFTDRAASKNRPALVLSDASAFNTPAGHVVLAMITSAKNPSWPLDCAIEDLAAAGLPAPSVVRCKLF
TLDARLIRGVLGVLAETDAQRTAQTLAQLLGSYS

Nucleotide

• Length: 345 bp

>T209223 NZ_CP103972:c1995098-1994802 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8319.39 Da
• Isoelectric Point: 4.4000

>AT209223 WP_218796620.1 NZ_CP079097:1820363-1820593 [Methylococcus capsulatus]
MTAVAKITAKGQTTIPAEIRAALHVSPGDLIAWEIEPDGSARVRRVQPLDIEYLKAVEGTLSEWTSPADEEAYRDL

Nucleotide

• Length: 231 bp

>AT209223 NZ_CP103972:c1994800-1994405 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA