Detailed information of TA system TA209216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 1590689..1591797
• Replicon: chromosome
• Accession: NZ_CP079095
• Organism: Methylococcus sp. Mc7

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KW115_RS07880
• Protein ID: WP_218808582.1
• Coordinates: 1590689..1591441 (-)
• Length: 251 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KW115_RS07885
• Protein ID: WP_218808583.1
• Coordinates: 1591438..1591797 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW115_RS07870 (KW115_07870)	1586466..1588013	-	1548	WP_218808581.1	DUF839 domain-containing protein	-
KW115_RS07875 (KW115_07875)	1589010..1590443	+	1434	WP_218807097.1	IS1380 family transposase	-
KW115_RS07880 (KW115_07880)	1590689..1591441	-	753	WP_218808582.1	RES family NAD+ phosphorylase	Toxin
KW115_RS07885 (KW115_07885)	1591438..1591797	-	360	WP_218808583.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
KW115_RS07890 (KW115_07890)	1592000..1592344	+	345	WP_218808584.1	phenylpyruvate tautomerase MIF-related protein	-
KW115_RS07895 (KW115_07895)	1592398..1593237	-	840	WP_218809008.1	shikimate dehydrogenase	-
KW115_RS07900 (KW115_07900)	1593234..1594238	-	1005	WP_218808585.1	porphobilinogen synthase	-
KW115_RS07905 (KW115_07905)	1594352..1595005	+	654	WP_218808586.1	ABC transporter substrate-binding protein	-
KW115_RS07910 (KW115_07910)	1595000..1595914	-	915	WP_218808587.1	oxygen-dependent coproporphyrinogen oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-206)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-117)

Sequences

Toxin

Protein

• Length: 251 a.a.
• Molecular weight: 27896.63 Da
• Isoelectric Point: 5.3200

>T209216 WP_218808582.1 NZ_CP079095:c1591441-1590689 [Methylococcus sp. Mc7]
MSAIWAACRDQAPLGPLHGHLYRMVESQEQVATYRIVSSLEEQALLEELLETSKPPPPETAGDFHYLLTTPFRYPPLRHG
SRFGRRHEPGLFYGSATVDTMLAEVAYYRFVFWQGMETPPQTPIVTQHTVFSARYACGRGLKLQDPPFDAWRATLTDPRH
YAPTQDLGSAMREAGVQAFEYLSARDPAAGINVALFEPAALASQRPIECQNWLAETSGEHVAFLGEARQIRGFELETFLV
EGMLPAPAVC

Nucleotide

• Length: 753 bp

>T209216 NZ_CP103972:c318849-318562 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 12942.99 Da
• Isoelectric Point: 10.6082

>AT209216 WP_218808583.1 NZ_CP079095:c1591797-1591438 [Methylococcus sp. Mc7]
MNTVAKAKPEPSSVLGHAFLEAGRKLGLSQSVLEVTIARNRSSISRNGIDPDSKSGELALLLIRIYRSLFALMGGDEAQM
AHWMKTRNRHTGGIPAEQIRSVQGLVGVVEYLDAMRGKI

Nucleotide

• Length: 360 bp

>AT209216 NZ_CP103972:c319088-318849 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA