Detailed information of TA system TA209206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2081789..2082318
• Replicon: chromosome
• Accession: NZ_CP078521
• Organism: Staphylococcus aureus strain ATCC 29213

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KWH15_RS10330
• Protein ID: WP_000621175.1
• Coordinates: 2081789..2082151 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KWH15_RS10335
• Protein ID: WP_000948331.1
• Coordinates: 2082148..2082318 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWH15_RS10310 (2078767)	2078767..2079537	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
KWH15_RS10315 (2079512)	2079512..2079991	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
KWH15_RS10320 (2079993)	2079993..2080319	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KWH15_RS10325 (2080438)	2080438..2081439	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KWH15_RS10330 (2081789)	2081789..2082151	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KWH15_RS10335 (2082148)	2082148..2082318	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KWH15_RS10340 (2082403)	2082403..2083551	-	1149	WP_001281154.1	alanine racemase	-
KWH15_RS10345 (2083617)	2083617..2083976	-	360	WP_000581197.1	holo-ACP synthase	-
KWH15_RS10350 (2083980)	2083980..2084471	-	492	WP_001205912.1	PH domain-containing protein	-
KWH15_RS10355 (2084458)	2084458..2086041	-	1584	WP_001294626.1	PH domain-containing protein	-
KWH15_RS10360 (2086034)	2086034..2086513	-	480	WP_001287087.1	hypothetical protein	-
KWH15_RS10365 (2086721)	2086721..2087281	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T209206 WP_000621175.1 NZ_CP078521:c2082151-2081789 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T209206 NZ_CP103964:1721937-1722392 [Staphylococcus hyicus]
ATGGCTAAGTATGAAGATTTAATGATACAAAACAGTCATATACCTATCAGTGATGAATATAGCCTAAAGCGTAACTTTAA
GGGGATATATGCAAACGGGGTAATACTGATTGATAAGAATTTAAGTAATGCTGAAAAGCATGAAGTTTTATCAGAAGAAC
TAGCACACCATAAACTTACATACGGTAACATTTTAGATCAATCAAACATGCTTAATCGAAAATTTGAATTAAAAGCAAGA
CGCCTTGCAAATGAATCAGTGATTACTTTGCAGGGTCTAATTAACGCTTTTAATTATGGTGTGCAAAATATTTATGATTT
AGCTACATATTTTGAAGTCACTAAAGATTTTGTACTAGATGCTATACAACATTACAAGCAAAAATATGGCTTAAGAACTC
GATATGGTAAATACATCATTGAGTTTGAACCGCTGATAATATATAAAGATTTATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT209206 WP_000948331.1 NZ_CP078521:c2082318-2082148 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT209206 NZ_CP103964:1721604-1721924 [Staphylococcus hyicus]
ATGAGAAATAATGACGAAATCATAAGTTTAGTAGCTAAAATAATGGAGCAAAATAATTTAAGTACTAGCGAATTAGCAAG
ACGTTTGAACATTGCGAAGTCATCTGTATCTCGTTATTTAAATAGAACTTCTCAATTCCCATTAAATAAAGTGAACGATT
TCGCTCATGCTTTAAATGTCACTCCTGAGTATTTACTAGGGGTTGAGGAAAATAAACAAAATACTTTAGCAGCACATCTC
GACGGAGACTTCACAGAAGAAGAATTGCAAGAAATTCTAGAGTATGCAGAGTTTGTAAAACAAAAACATAGAAACAAATA
A

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1