Detailed information of TA system TA209189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 62606..63177
• Replicon: plasmid pNS2A
• Accession: NZ_CP078163
• Organism: Enterococcus faecalis strain NS2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2Z2CGM4
• Locus tag: KVY10_RS12875
• Protein ID: WP_002362432.1
• Coordinates: 62606..62947 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: KVY10_RS12880
• Protein ID: WP_002362431.1
• Coordinates: 62947..63177 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVY10_RS12855 (KVY10_12855)	58005..59288	-	1284	Protein_70	hypothetical protein	-
KVY10_RS12865 (KVY10_12865)	60689..61291	-	603	WP_002362434.1	Fic family protein	-
KVY10_RS12870 (KVY10_12870)	61556..62494	-	939	WP_002362433.1	hypothetical protein	-
KVY10_RS12875 (KVY10_12875)	62606..62947	-	342	WP_002362432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KVY10_RS12880 (KVY10_12880)	62947..63177	-	231	WP_002362431.1	hypothetical protein	Antitoxin
KVY10_RS12885 (KVY10_12885)	63381..64001	+	621	WP_002367784.1	recombinase family protein	-
KVY10_RS12890 (KVY10_12890)	63991..64305	+	315	WP_002367785.1	hypothetical protein	-
KVY10_RS13070	64299..64505	+	207	WP_002367786.1	hypothetical protein	-
KVY10_RS12895 (KVY10_12895)	64665..64859	+	195	WP_002367787.1	hypothetical protein	-
KVY10_RS12900 (KVY10_12900)	64871..65062	+	192	WP_002367788.1	hypothetical protein	-
KVY10_RS12905 (KVY10_12905)	65232..65447	+	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
KVY10_RS12910 (KVY10_12910)	65448..65789	+	342	WP_002367792.1	bacteriocin immunity protein	-
KVY10_RS12915 (KVY10_12915)	66205..66723	+	519	WP_002367793.1	hypothetical protein	-
KVY10_RS13075	66671..66886	+	216	WP_002415356.1	hypothetical protein	-
KVY10_RS12920 (KVY10_12920)	66978..67064	+	87	WP_012881081.1	type I toxin-antitoxin system Fst family toxin	-
KVY10_RS12925 (KVY10_12925)	67321..67617	+	297	WP_002367795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aph(3')-III / dfrG / cat / tet(L) / tet(M) / fexA / optrA	-	1..77697	77697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13244.44 Da
• Isoelectric Point: 8.0113

>T209189 WP_002362432.1 NZ_CP078163:c62947-62606 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T209189 NZ_CP103894:c995440-995177 [Corynebacterium diphtheriae bv. mitis]
GTGGCTTGGGAGATTAGTTTTTCTCCTCGGGCGGTGAAGTCGTTTAAGAAGCTCGATACGGGTGAGCAGCGGAGGGTGAG
TAAGTTTCTGCGGGAGGTTGGCGCCCTTGAGGACCCGAGGTTACGAGGTAAGGCATTAACAGCCAATAAGTCGGGTTTGT
GGCGCTGGCGTGTGGGGGACTATCGGATTATTGCTGACATTGTGGACGCGCGTGTTGTCGTCGTTGTGGTTGACGTTGGG
CACCGCTCGAAGGTCTACGACTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT209189 WP_002362431.1 NZ_CP078163:c63177-62947 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT209189 NZ_CP103894:c995660-995424 [Corynebacterium diphtheriae bv. mitis]
ATGAGTGGTTCGGTGATTAGCTTGCGTATCGATGACGAGCAGAAGCGGCGTTTGGATGAGCTGTCGCGACGTACGGGGCG
GCCGTGTTCGTTTTATCTTCGGGAAGCGTTGAGTGCACATCTGGCTGATCTGGAGTATGTGTATCGCTTGGAGGAGGAGG
CTGCTCAGGTTCGTCGTGGGGAGTTGGGAACTGTGCCGTTGAGTCAGCTGGAGCGCGAGTGTGGCTTGGGAGATTAG

Structures

Toxin

• AlphaFold DB: A0A2Z2CGM4

Antitoxin

• AlphaFold DB: R3KHK9