Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 5936..7073 Replicon plasmid pNS2A
Accession NZ_CP078163
Organism Enterococcus faecalis strain NS2

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag KVY10_RS12555 Protein ID WP_002332783.1
Coordinates 5936..6799 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag KVY10_RS12560 Protein ID WP_002326825.1
Coordinates 6801..7073 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KVY10_RS12520 (KVY10_12520) 1033..1116 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
KVY10_RS12525 (KVY10_12525) 1658..2451 - 794 Protein_3 aminoglycoside O-phosphotransferase APH(3')-IIIa -
KVY10_RS12530 (KVY10_12530) 2544..3086 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
KVY10_RS12535 (KVY10_12535) 3083..3589 - 507 Protein_5 aminoglycoside 6-adenylyltransferase -
KVY10_RS12540 (KVY10_12540) 3664..4344 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
KVY10_RS12545 (KVY10_12545) 4378..4884 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
KVY10_RS12550 (KVY10_12550) 5180..5497 - 318 WP_002326830.1 hypothetical protein -
KVY10_RS12555 (KVY10_12555) 5936..6799 - 864 WP_002332783.1 zeta toxin family protein Toxin
KVY10_RS12560 (KVY10_12560) 6801..7073 - 273 WP_002326825.1 antitoxin Antitoxin
KVY10_RS12565 (KVY10_12565) 7091..7306 - 216 WP_001835296.1 peptide-binding protein -
KVY10_RS12570 (KVY10_12570) 7398..8294 - 897 WP_002326827.1 ParA family protein -
KVY10_RS12575 (KVY10_12575) 8397..8657 - 261 Protein_13 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
KVY10_RS12580 (KVY10_12580) 8827..9564 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
KVY10_RS12585 (KVY10_12585) 9689..9772 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
KVY10_RS12590 (KVY10_12590) 10204..10575 - 372 WP_002358205.1 hypothetical protein -
KVY10_RS12595 (KVY10_12595) 10568..11413 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IS/Tn erm(B) / aph(3')-III / dfrG - 171..9564 9393
- inside Conjugative plasmid erm(B) / aph(3')-III / dfrG / cat / tet(L) / tet(M) / fexA / optrA - 1..77697 77697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T209188 WP_002332783.1 NZ_CP078163:c6799-5936 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T209188 NZ_CP103894:378918-379337 [Corynebacterium diphtheriae bv. mitis]
ATGGTGAACAAGTTGGTCTACTTATCCTGCACCTTAAATCAGGAGTGCGGGAAGCGAACAGGTCCTCACAGAGAACCTGA
ACCTTTTCTTGGATTCTCCGTGAGGGATCGGAGTGGGCTAGAGGGCGCGATCGCTTCAGTATTTCAATCGTCGTTTGGCG
TCTTGCTGTACCCGACCATCTACGACCAAGCGGCCGCACTCCTGTTCAAGCTGGCAAACGGGCATTACTTCCAAGACGGA
AACAAGCGAACAGCATTCCACCTGTGCTTAGCCTACCTACGGGCCAATAACGTTCACGTCAGTCCCCCTCGGTCTGATGA
CGTCATCACTTTACTTCAGACCACGGTGAGTCGCTCAGGAAGCCAAGAAAACGGGATAGCGTTCGTTAGACAAAAGCTTT
TGGACTGGACCGTAGACTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT209188 WP_002326825.1 NZ_CP078163:c7073-6801 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT209188 NZ_CP103894:378307-378873 [Corynebacterium diphtheriae bv. mitis]
ATGATCAGCATCCACGATGTTGCAGCGCGTGTACTTGAGCTGCACCCTTCCTGCGACAAGATGAAGCTGTACAAACTGTG
TTACTTCTCCCAGGGGTGGCACTTGGCATGGACAGGTCGTCCACTATTCAACGAAGAGCTACAGGCGTGGAAGTACGGGG
CCGTATCTCCTACTTTGCGTCAGGCTAGCGAGCTTCGTGCCGATGATGACCGTCGAGTGACCCAAATTTGGAGCGGGGAC
AGTTCTCAACTCACCGATTACGAGCGGTCAGTCGTGGACACTGTTGTTTCGTTCTATGGAGATCTTGGGTCTTTTCACCT
GTCCGATTTAAGCCACGGCTTCGCCTGGTCCACCGTACGAGGCGACCTTCCTCCAGACGCCTCGTGCAGCGATGTGATCC
CTCACTCTCTGATCCGTCGTGAGTTTGTCGAGAATGCTTGGGGCGAAGCTCCTACGCCAAACGCTCCTTCACACCTGCCT
TCCATGGCACTTGATTCTCTCGAGCAGGCAGCCGATGACGTTGCTGCCGAAAATGCAGAGACGCTTCGACTTCTCGCTTT
CATCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References