Detailed information of TA system TA209169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2555536..2556107
• Replicon: chromosome
• Accession: NZ_CP078162
• Organism: Enterococcus faecalis strain NS2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KVY10_RS12030
• Protein ID: WP_002354774.1
• Coordinates: 2555536..2555877 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: KVY10_RS12035
• Protein ID: WP_002354773.1
• Coordinates: 2555877..2556107 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVY10_RS12025 (2551551)	2551551..2555165	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
KVY10_RS12030 (2555536)	2555536..2555877	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KVY10_RS12035 (2555877)	2555877..2556107	-	231	WP_002354773.1	hypothetical protein	Antitoxin
KVY10_RS12040 (2556514)	2556514..2556729	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
KVY10_RS12045 (2556868)	2556868..2557860	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
KVY10_RS12050 (2558124)	2558124..2558762	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
KVY10_RS12055 (2559449)	2559449..2561065	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T209169 WP_002354774.1 NZ_CP078162:c2555877-2555536 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T209169 NZ_CP103871:c201956-201609 [Actinacidiphila bryophytorum]
ATGCTGCACCAGGACGTCCACGACTGGTACCTGGACCTCGCCCGGAAGGACCCCGAGTCCGCGCATCTGGTCGAGCAGGC
CATCGACCTGCTTGCCGACACGGGGCCGACGCTGGGTCGTCCCCTGGCGGACAGGGTCAGCGGTTCCAGGCACCACAACA
TGAAGGAGTTACGCCCTGGCTCCGTCGGCGCCACGGAGATCCGCATACTGTTCGCGTTTGACCCGCAGCGGCAAGCGCTC
ATCCTGGTGGCCGGAGACAAGAGCGGCTCGTGGAACAGCTGGTACGACCGGAACATCCCGGTCGCGGACGACCGGTTCGA
CGAGCACCTCGACACGTTGGACGGGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT209169 WP_002354773.1 NZ_CP078162:c2556107-2555877 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT209169 NZ_CP103871:c201556-201227 [Actinacidiphila bryophytorum]
ATGCACAGCTGGAAGGACACCAAGGCCGAGGCCGACGCCGTCCACGAGGCGAACGGCACGCGCGCCACCGGCGAGCAGAA
GGCCGCGATCCGCGAGGAGCTCGAAGGCCGGGTGCGTGCCTACCGCCTCGCGGAGATCCGCGAGGAGCAGGCCCTCACCC
AGACCGATGTCGCCAAGGCGATGGGGACGACCCAGTCGAACGTGTCCCGCTTCGAACGGGGCGAGCTGGGGCGGTCGGAA
CTGGCCACGATCGCCGCTTACGTGGAGGCGCTCGGCGGCACGCTGCGGCTCGTGGCGGATTTCGGCGACCGGCAGCTTCG
GGTCAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812