Detailed information of TA system TA209148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 839094..839626
• Replicon: chromosome
• Accession: NZ_CP078159
• Organism: Staphylococcus haemolyticus strain NY5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q4L7V3
• Locus tag: KVY09_RS04090
• Protein ID: WP_011275272.1
• Coordinates: 839261..839626 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KVY09_RS04085
• Protein ID: WP_049426542.1
• Coordinates: 839094..839264 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVY09_RS04060 (KVY09_04060)	834880..835359	+	480	WP_053020220.1	PH domain-containing protein	-
KVY09_RS04065 (KVY09_04065)	835352..836875	+	1524	WP_049426546.1	PH domain-containing protein	-
KVY09_RS04070 (KVY09_04070)	836868..837395	+	528	WP_049426545.1	PH domain-containing protein	-
KVY09_RS04075 (KVY09_04075)	837405..837764	+	360	WP_049426544.1	holo-ACP synthase	-
KVY09_RS04080 (KVY09_04080)	837860..839008	+	1149	WP_070440784.1	alanine racemase	-
KVY09_RS04085 (KVY09_04085)	839094..839264	+	171	WP_049426542.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KVY09_RS04090 (KVY09_04090)	839261..839626	+	366	WP_011275272.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KVY09_RS04095 (KVY09_04095)	839934..840935	+	1002	WP_053020219.1	PP2C family protein-serine/threonine phosphatase	-
KVY09_RS04100 (KVY09_04100)	841034..841360	+	327	WP_049426540.1	anti-sigma factor antagonist	-
KVY09_RS04105 (KVY09_04105)	841389..841841	+	453	WP_046309853.1	anti-sigma B factor RsbW	-
KVY09_RS04110 (KVY09_04110)	841816..842586	+	771	WP_070440787.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13672.75 Da
• Isoelectric Point: 8.4121

>T209148 WP_011275272.1 NZ_CP078159:839261-839626 [Staphylococcus haemolyticus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYRLDKDSVILLEQIR
TLDKKRLKEKLTYLSDEKMQEVDEALDISLGLHDEVKTQNT

Nucleotide

• Length: 366 bp

>T209148 NZ_CP103864:1580663-1581148 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6396.08 Da
• Isoelectric Point: 4.0325

>AT209148 WP_049426542.1 NZ_CP078159:839094-839264 [Staphylococcus haemolyticus]
MLTFNQNRSHSLEQSLKEGYSQMAELNLSLATEAFPLECEACDCNETYIISNYKSE

Nucleotide

• Length: 171 bp

>AT209148 NZ_CP103864:1580394-1580672 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A2A1KA75