Detailed information of TA system TA209146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1241205..1242122
• Replicon: chromosome
• Accession: NZ_CP078149
• Organism: Bacillus velezensis strain BS-G1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: KV103_RS06435
• Protein ID: WP_007407256.1
• Coordinates: 1241376..1242122 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KV103_RS06430
• Protein ID: WP_003154807.1
• Coordinates: 1241205..1241375 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KV103_RS06395	1236438..1238060	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
KV103_RS06400	1238073..1238444	+	372	WP_032874603.1	XkdW family protein	-
KV103_RS06405	1238449..1238646	+	198	WP_032874605.1	XkdX family protein	-
KV103_RS06410	1238703..1239464	+	762	WP_032874607.1	hypothetical protein	-
KV103_RS06415	1239516..1239779	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KV103_RS06420	1239793..1240056	+	264	WP_003154813.1	phage holin	-
KV103_RS06425	1240070..1240948	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
KV103_RS19440	1240983..1241108	-	126	WP_003154809.1	hypothetical protein	-
KV103_RS06430	1241205..1241375	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KV103_RS06435	1241376..1242122	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KV103_RS06440	1242227..1243225	-	999	WP_032874611.1	inorganic phosphate transporter	-
KV103_RS06445	1243238..1243855	-	618	WP_032874614.1	DUF47 domain-containing protein	-
KV103_RS06450	1244141..1245457	-	1317	WP_032874616.1	amino acid permease	-
KV103_RS06455	1245779..1246729	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1194267..1299974	105707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T209146 WP_007407256.1 NZ_CP078149:c1242122-1241376 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T209146 NZ_CP103864:c1286515-1286201 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT209146 WP_003154807.1 NZ_CP078149:c1241375-1241205 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT209146 NZ_CP103864:c1286204-1285788 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14