Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 129003..129739 Replicon plasmid pJNQH579-1
Accession NZ_CP078147
Organism Klebsiella variicola strain JNQH579

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag KW408_RS27645 Protein ID WP_049083183.1
Coordinates 129003..129485 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag KW408_RS27650 Protein ID WP_049083184.1
Coordinates 129473..129739 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW408_RS27620 (KW408_27620) 124918..125427 - 510 WP_218408483.1 DUF2778 domain-containing protein -
KW408_RS27625 (KW408_27625) 125693..126766 + 1074 WP_004118212.1 FUSC family protein -
KW408_RS27630 (KW408_27630) 126874..127284 - 411 Protein_126 C-terminal helicase domain-containing protein -
KW408_RS27640 (KW408_27640) 128053..128796 - 744 Protein_128 ISNCY family transposase -
KW408_RS27645 (KW408_27645) 129003..129485 - 483 WP_049083183.1 GNAT family N-acetyltransferase Toxin
KW408_RS27650 (KW408_27650) 129473..129739 - 267 WP_049083184.1 DUF1778 domain-containing protein Antitoxin
KW408_RS27655 (KW408_27655) 129826..130158 + 333 WP_218408484.1 hypothetical protein -
KW408_RS27660 (KW408_27660) 130211..130993 - 783 WP_110184587.1 IS21-like element ISSen3 family helper ATPase IstB -
KW408_RS27665 (KW408_27665) 130990..132012 - 1023 WP_148842283.1 IS21 family transposase -
KW408_RS27670 (KW408_27670) 132102..132566 + 465 WP_177957707.1 hypothetical protein -
KW408_RS27680 (KW408_27680) 133473..134441 - 969 Protein_136 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..197434 197434
- inside IScluster/Tn - - 122474..134195 11721


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17326.90 Da        Isoelectric Point: 9.1188

>T209144 WP_049083183.1 NZ_CP078147:c129485-129003 [Klebsiella variicola]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T209144 NZ_CP103864:c946097-945762 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10117.61 Da        Isoelectric Point: 4.6437

>AT209144 WP_049083184.1 NZ_CP078147:c129739-129473 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT209144 NZ_CP103864:c946345-946097 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References