Detailed information of TA system TA209144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 129003..129739
• Replicon: plasmid pJNQH579-1
• Accession: NZ_CP078147
• Organism: Klebsiella variicola strain JNQH579

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KW408_RS27645
• Protein ID: WP_049083183.1
• Coordinates: 129003..129485 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: KW408_RS27650
• Protein ID: WP_049083184.1
• Coordinates: 129473..129739 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW408_RS27620 (KW408_27620)	124918..125427	-	510	WP_218408483.1	DUF2778 domain-containing protein	-
KW408_RS27625 (KW408_27625)	125693..126766	+	1074	WP_004118212.1	FUSC family protein	-
KW408_RS27630 (KW408_27630)	126874..127284	-	411	Protein_126	C-terminal helicase domain-containing protein	-
KW408_RS27640 (KW408_27640)	128053..128796	-	744	Protein_128	ISNCY family transposase	-
KW408_RS27645 (KW408_27645)	129003..129485	-	483	WP_049083183.1	GNAT family N-acetyltransferase	Toxin
KW408_RS27650 (KW408_27650)	129473..129739	-	267	WP_049083184.1	DUF1778 domain-containing protein	Antitoxin
KW408_RS27655 (KW408_27655)	129826..130158	+	333	WP_218408484.1	hypothetical protein	-
KW408_RS27660 (KW408_27660)	130211..130993	-	783	WP_110184587.1	IS21-like element ISSen3 family helper ATPase IstB	-
KW408_RS27665 (KW408_27665)	130990..132012	-	1023	WP_148842283.1	IS21 family transposase	-
KW408_RS27670 (KW408_27670)	132102..132566	+	465	WP_177957707.1	hypothetical protein	-
KW408_RS27680 (KW408_27680)	133473..134441	-	969	Protein_136	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..197434	197434
	-	inside	IScluster/Tn	-	-	122474..134195	11721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17326.90 Da
• Isoelectric Point: 9.1188

>T209144 WP_049083183.1 NZ_CP078147:c129485-129003 [Klebsiella variicola]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T209144 NZ_CP103864:c946097-945762 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10117.61 Da
• Isoelectric Point: 4.6437

>AT209144 WP_049083184.1 NZ_CP078147:c129739-129473 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT209144 NZ_CP103864:c946345-946097 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA