Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5470572..5471197 Replicon chromosome
Accession NZ_CP078146
Organism Klebsiella variicola strain JNQH579

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag KW408_RS26410 Protein ID WP_008807903.1
Coordinates 5470814..5471197 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KW408_RS26405 Protein ID WP_004150355.1
Coordinates 5470572..5470814 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW408_RS26380 (KW408_26380) 5466562..5467161 + 600 WP_008807908.1 glucose-1-phosphatase -
KW408_RS26385 (KW408_26385) 5467155..5468015 + 861 WP_008807907.1 virulence factor BrkB family protein -
KW408_RS26390 (KW408_26390) 5468012..5468449 + 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
KW408_RS26395 (KW408_26395) 5468494..5469435 + 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
KW408_RS26400 (KW408_26400) 5469450..5470367 - 918 WP_049155024.1 alpha/beta hydrolase -
KW408_RS26405 (KW408_26405) 5470572..5470814 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KW408_RS26410 (KW408_26410) 5470814..5471197 + 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
KW408_RS26415 (KW408_26415) 5471371..5472300 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KW408_RS26420 (KW408_26420) 5472297..5472932 - 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
KW408_RS26425 (KW408_26425) 5472929..5473831 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209142 WP_008807903.1 NZ_CP078146:5470814-5471197 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T209142 NZ_CP103863:1043597-1043737 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209142 WP_004150355.1 NZ_CP078146:5470572-5470814 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209142 NZ_CP103863:c1043925-1043670 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACAAT
GGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTTTTGATTATTTAA
AAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGTGCAATCAAAGCA
ATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References