Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5113597..5114228 Replicon chromosome
Accession NZ_CP078146
Organism Klebsiella variicola strain JNQH579

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag KW408_RS24800 Protein ID WP_022066423.1
Coordinates 5113953..5114228 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag KW408_RS24795 Protein ID WP_004181489.1
Coordinates 5113597..5113956 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW408_RS24770 (KW408_24770) 5109854..5110798 + 945 WP_008806948.1 nickel ABC transporter permease subunit NikB -
KW408_RS24775 (KW408_24775) 5110795..5111628 + 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
KW408_RS24780 (KW408_24780) 5111628..5112392 + 765 WP_117116351.1 nickel import ATP-binding protein NikD -
KW408_RS24785 (KW408_24785) 5112389..5113180 + 792 WP_012540373.1 nickel import ATP-binding protein NikE -
KW408_RS24790 (KW408_24790) 5113168..5113569 + 402 WP_048330886.1 nickel-responsive transcriptional regulator NikR -
KW408_RS24795 (KW408_24795) 5113597..5113956 - 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KW408_RS24800 (KW408_24800) 5113953..5114228 - 276 WP_022066423.1 type II toxin-antitoxin system HicA family toxin Toxin
KW408_RS24805 (KW408_24805) 5114625..5115638 + 1014 WP_211743759.1 magnesium transporter -
KW408_RS24810 (KW408_24810) 5115678..5116871 - 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
KW408_RS24815 (KW408_24815) 5117101..5118597 + 1497 WP_008806938.1 inorganic phosphate transporter PitA -
KW408_RS24820 (KW408_24820) 5118657..5118992 - 336 WP_002921203.1 universal stress protein UspB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10267.85 Da        Isoelectric Point: 10.3165

>T209141 WP_022066423.1 NZ_CP078146:c5114228-5113953 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T209141 NZ_CP103863:1043597-1043737 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT209141 WP_004181489.1 NZ_CP078146:c5113956-5113597 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT209141 NZ_CP103863:c1043595-1043452 [Enterococcus faecalis]
TGCTACAATAGAGATGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References