Detailed information of TA system TA209138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3770683..3771680
• Replicon: chromosome
• Accession: NZ_CP078146
• Organism: Klebsiella variicola strain JNQH579

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KW408_RS30095
• Protein ID: WP_229531768.1
• Coordinates: 3770683..3771237 (+)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1F2MDT5
• Locus tag: KW408_RS18180
• Protein ID: WP_012541033.1
• Coordinates: 3771234..3771680 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW408_RS18150 (KW408_18150)	3766908..3767312	+	405	WP_004201620.1	CidA/LrgA family protein	-
KW408_RS18155 (KW408_18155)	3767302..3767997	+	696	WP_008804035.1	CidB/LrgB family autolysis modulator	-
KW408_RS18160 (KW408_18160)	3768126..3769010	+	885	WP_218408152.1	cytidine deaminase	-
KW408_RS18165 (KW408_18165)	3769032..3769250	+	219	WP_071583089.1	hypothetical protein	-
KW408_RS18170 (KW408_18170)	3769287..3770018	+	732	WP_008804033.1	outer membrane permeability protein SanA	-
KW408_RS30090	3770133..3770654	+	522	WP_228744119.1	phosphatidylinositol kinase	-
KW408_RS30095	3770683..3771237	+	555	WP_229531768.1	HipA domain-containing protein	Toxin
KW408_RS18180 (KW408_18180)	3771234..3771680	+	447	WP_012541033.1	helix-turn-helix transcriptional regulator	Antitoxin
KW408_RS18185 (KW408_18185)	3771722..3772732	-	1011	WP_002912871.1	galactose/methyl galactoside ABC transporter permease MglC	-
KW408_RS18190 (KW408_18190)	3772748..3774268	-	1521	WP_008804029.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
KW408_RS18195 (KW408_18195)	3774387..3775385	-	999	WP_008804028.1	galactose/glucose ABC transporter substrate-binding protein MglB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	wbtL / ugd / wbtL / gnd / wza / wzi / cpsACP / galF	3603002..3807102	204100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (2-147)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20710.03 Da
• Isoelectric Point: 5.7891

>T209138 WP_229531768.1 NZ_CP078146:3770683-3771237 [Klebsiella variicola]
MRLGMESVYSLLQKAPATILDHETTLRILIEKITSSNMVRVQNYRFDVQGFIIDWVRRDLLNIIFGNSDNHGRNTAFMKA
DNEIILAPIYDFAPMKADPEGIPRTMKWSLACESGGEYNFGAIAQALAEWITPDTLLNALSETASQLVDLPSRLKVRGVP
VQILEMPSIGFKFIPDKLARWGLL

Nucleotide

• Length: 555 bp

>T209138 NZ_CP103863:c893222-893121 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16396.05 Da
• Isoelectric Point: 10.5417

>AT209138 WP_012541033.1 NZ_CP078146:3771234-3771680 [Klebsiella variicola]
MKTLNKKETEIQNTLARLRADNPPSLPATGVTATEKKARSEISRQRVSAGLKKVKTVDRQAVIHAIIHDIMLGAISQGEA
LKKLRVEVLGLRQDEYARLVDVSRKTLSDVENDKGNYSAEIINKIYKPFGLETGLVPISKTLISSLFK

Nucleotide

• Length: 447 bp

>AT209138 NZ_CP103863:892997-893176 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2MDT5