Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3563010..3563600 Replicon chromosome
Accession NZ_CP078146
Organism Klebsiella variicola strain JNQH579

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag KW408_RS17260 Protein ID WP_008804165.1
Coordinates 3563010..3563342 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A8E1V487
Locus tag KW408_RS17265 Protein ID WP_041909206.1
Coordinates 3563343..3563600 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW408_RS17235 (KW408_17235) 3559047..3559292 + 246 WP_008804171.1 signal transduction protein PmrD -
KW408_RS17240 (KW408_17240) 3559423..3560877 + 1455 WP_008804170.1 AMP nucleosidase -
KW408_RS17250 (KW408_17250) 3561251..3562687 - 1437 WP_164728680.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
KW408_RS17260 (KW408_17260) 3563010..3563342 - 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KW408_RS17265 (KW408_17265) 3563343..3563600 - 258 WP_041909206.1 antitoxin Antitoxin
KW408_RS17270 (KW408_17270) 3564079..3565199 + 1121 WP_218408042.1 IS3 family transposase -
KW408_RS17275 (KW408_17275) 3565418..3566645 + 1228 WP_218408074.1 IS3 family transposase -
KW408_RS17280 (KW408_17280) 3566620..3567315 + 696 WP_228744110.1 hypothetical protein -
KW408_RS17285 (KW408_17285) 3567424..3568404 - 981 WP_000019402.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T209137 WP_008804165.1 NZ_CP078146:c3563342-3563010 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T209137 NZ_CP103863:c892789-892688 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9449.95 Da        Isoelectric Point: 4.6234

>AT209137 WP_041909206.1 NZ_CP078146:c3563600-3563343 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT209137 NZ_CP103863:892566-892743 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCTTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure

References