Detailed information of TA system TA209104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 59010..59625
• Replicon: plasmid pPsv_PVFi1
• Accession: NZ_CP078140
• Organism: Pseudomonas savastanoi pv. savastanoi strain PVFi1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HQ966_RS28785
• Protein ID: WP_080719041.1
• Coordinates: 59470..59625 (-)
• Length: 52 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HQ966_RS28780
• Protein ID: WP_054082441.1
• Coordinates: 59010..59438 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ966_RS28740 (HQ966_28530)	54353..54607	-	255	WP_010208566.1	hypothetical protein	-
HQ966_RS28745 (HQ966_28535)	54692..54967	+	276	Protein_61	hypothetical protein	-
HQ966_RS28750 (HQ966_28540)	54976..55146	+	171	Protein_62	IS630 family transposase	-
HQ966_RS28755 (HQ966_28545)	55457..56491	+	1035	WP_015060660.1	IS110-like element ISPsy16 family transposase	-
HQ966_RS28760 (HQ966_28550)	56868..57026	+	159	WP_237901257.1	MobA/MobL family protein	-
HQ966_RS28765 (HQ966_28555)	57023..57268	-	246	WP_237901249.1	hypothetical protein	-
HQ966_RS28775 (HQ966_28565)	58623..58844	-	222	WP_235208930.1	hypothetical protein	-
HQ966_RS28780 (HQ966_28570)	59010..59438	-	429	WP_054082441.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HQ966_RS28785 (HQ966_28575)	59470..59625	-	156	WP_080719041.1	type II toxin-antitoxin system HicA family toxin	Toxin
HQ966_RS28790 (HQ966_28580)	60113..60586	+	474	WP_074800939.1	DUF3757 domain-containing protein	-
HQ966_RS28795 (HQ966_28585)	60840..61139	+	300	WP_160188679.1	FCD domain-containing protein	-
HQ966_RS28800 (HQ966_28590)	61174..61962	+	789	WP_002556064.1	amino acid ABC transporter ATP-binding protein	-
HQ966_RS28805 (HQ966_28595)	61984..62898	+	915	WP_002556065.1	aminotransferase class IV	-
HQ966_RS28810 (HQ966_28600)	62925..63761	+	837	WP_031598553.1	transporter substrate-binding domain-containing protein	-
HQ966_RS28815 (HQ966_28605)	63826..64488	+	663	WP_031598554.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..72349	72349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-49)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5640.61 Da
• Isoelectric Point: 10.6276

>T209104 WP_080719041.1 NZ_CP078140:c59625-59470 [Pseudomonas savastanoi pv. savastanoi]
MQPQAALFKAAKGSHFKVYLNGKATIFADHGSKEMHEGLRKSIIKQLGLKD

Nucleotide

• Length: 156 bp

>T209104 NZ_CP103850:c1930193-1930041 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15893.28 Da
• Isoelectric Point: 5.1798

>AT209104 WP_054082441.1 NZ_CP078140:c59438-59010 [Pseudomonas savastanoi pv. savastanoi]
MYQYQYPLTQHQEQTGVWLSCPNIPEMNASGDTLTEALDEALNGMESALSLYVDQRRKIPQASLPVGDELVMHLPALTVA
KIMLWNSMLDNGVSRAELARRLGCTRQVVDRLVDFLHTSKIEQVERALGLLGRRITLSLEAA

Nucleotide

• Length: 429 bp

>AT209104 NZ_CP103850:c1930817-1930218 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA