Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 21838..22385 Replicon plasmid pPsv_PVFi1
Accession NZ_CP078140
Organism Pseudomonas savastanoi pv. savastanoi strain PVFi1

Toxin (Protein)


Gene name mazF Uniprot ID Q48B48
Locus tag HQ966_RS28525 Protein ID WP_003319696.1
Coordinates 22059..22385 (+) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID H1ZWZ3
Locus tag HQ966_RS28520 Protein ID WP_015060676.1
Coordinates 21838..22062 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQ966_RS28505 (HQ966_28305) 20179..20811 - 633 WP_015060648.1 recombinase family protein -
HQ966_RS28510 (HQ966_28310) 20852..21271 - 420 WP_031599341.1 type II toxin-antitoxin system VapC family toxin -
HQ966_RS28515 (HQ966_28315) 21268..21525 - 258 WP_003381346.1 Arc family DNA-binding protein -
HQ966_RS28520 (HQ966_28320) 21838..22062 + 225 WP_015060676.1 antitoxin MazE family protein Antitoxin
HQ966_RS28525 (HQ966_28325) 22059..22385 + 327 WP_003319696.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HQ966_RS28530 22597..23187 - 591 WP_230851525.1 protein-tyrosine phosphatase family protein -
HQ966_RS28535 23479..23997 - 519 Protein_19 restriction endonuclease -
HQ966_RS28540 (HQ966_28335) 24202..25377 + 1176 WP_237901253.1 IS91 family transposase -
HQ966_RS28545 (HQ966_28340) 25717..25890 + 174 WP_005759896.1 hypothetical protein -
HQ966_RS28550 (HQ966_28345) 25890..26495 + 606 WP_005759898.1 hypothetical protein -
HQ966_RS28555 (HQ966_28350) 26578..27210 - 633 WP_237901254.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..72349 72349


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Antitoxin MazE-like

(8-72)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11660.71 Da        Isoelectric Point: 8.0546

>T209102 WP_003319696.1 NZ_CP078140:22059-22385 [Pseudomonas savastanoi pv. savastanoi]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Download         Length: 327 bp

>T209102 NZ_CP103850:c841686-841498 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8515.62 Da        Isoelectric Point: 5.5294

>AT209102 WP_015060676.1 NZ_CP078140:21838-22062 [Pseudomonas savastanoi pv. savastanoi]
MPTSHVNDRVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Download         Length: 225 bp

>AT209102 NZ_CP103850:c841975-841715 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8S2J7

Antitoxin

Source ID Structure
AlphaFold DB A0A330JWK7

References