Detailed information of TA system TA209101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 20852..21525
• Replicon: plasmid pPsv_PVFi1
• Accession: NZ_CP078140
• Organism: Pseudomonas savastanoi pv. savastanoi strain PVFi1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HQ966_RS28510
• Protein ID: WP_031599341.1
• Coordinates: 20852..21271 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q48B52
• Locus tag: HQ966_RS28515
• Protein ID: WP_003381346.1
• Coordinates: 21268..21525 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ966_RS28505 (HQ966_28305)	20179..20811	-	633	WP_015060648.1	recombinase family protein	-
HQ966_RS28510 (HQ966_28310)	20852..21271	-	420	WP_031599341.1	type II toxin-antitoxin system VapC family toxin	Toxin
HQ966_RS28515 (HQ966_28315)	21268..21525	-	258	WP_003381346.1	Arc family DNA-binding protein	Antitoxin
HQ966_RS28520 (HQ966_28320)	21838..22062	+	225	WP_015060676.1	antitoxin MazE family protein	-
HQ966_RS28525 (HQ966_28325)	22059..22385	+	327	WP_003319696.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HQ966_RS28530	22597..23187	-	591	WP_230851525.1	protein-tyrosine phosphatase family protein	-
HQ966_RS28535	23479..23997	-	519	Protein_19	restriction endonuclease	-
HQ966_RS28540 (HQ966_28335)	24202..25377	+	1176	WP_237901253.1	IS91 family transposase	-
HQ966_RS28545 (HQ966_28340)	25717..25890	+	174	WP_005759896.1	hypothetical protein	-
HQ966_RS28550 (HQ966_28345)	25890..26495	+	606	WP_005759898.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..72349	72349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15100.38 Da
• Isoelectric Point: 4.8667

>T209101 WP_031599341.1 NZ_CP078140:c21271-20852 [Pseudomonas savastanoi pv. savastanoi]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIAFANGFTVATRDTGPFEAAGLNVINPWGA

Nucleotide

• Length: 420 bp

>T209101 NZ_CP103834:2050348-2050608 [Actinobacillus genomosp. 1]
ATGTTTGAATATGATCCTAATAAAAGTCGAATAAATAAAGAAAAGCATGGTATAGACTTTGAAGAAGCAAAACAATTATG
GAAAGATGAGCAATTGCTAACTTTTACTGTTATGAACGATCCTGAGCATAGGCTATTAGCTATTGGACAGATTAATAAAA
AACATTGGTCTGCAATTTTTACCGTTAGAGGTAAAAATGTACGTTTAATTTCAGTTAGACGTTCAAGAAAAAAAGAGGTA
GAGCTTTATGAAAACACATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9401.62 Da
• Isoelectric Point: 9.0330

>AT209101 WP_003381346.1 NZ_CP078140:c21525-21268 [Pseudomonas savastanoi pv. savastanoi]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Nucleotide

• Length: 258 bp

>AT209101 NZ_CP103834:2050595-2050837 [Actinobacillus genomosp. 1]
ATGAAAACACATAGTTTGGAATTTGAGCGGAAATTTGATGATAATGAGGAAGATATTGTTGAATATCTTGATTTAACAAG
TGCTAATCGCCCTAATCAAAAAAGTAAACGTGTCAATGTTGATTTCCCGATATGGATGGTTGAAAGTCTTGATAAAGAAG
CGCAACATTTAGGTGTTCCGCGGCAAGCATTGATTAAACTTTGGATTGCAGAAAAACTTCCAAAGCCTCATATAGCCTCT
TAA

Structures

Antitoxin

• AlphaFold DB: Q48B52