Detailed information of TA system TA209098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 4194498..4195171
• Replicon: chromosome
• Accession: NZ_CP078139
• Organism: Pseudomonas savastanoi pv. savastanoi strain PVFi1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HQ966_RS19090
• Protein ID: WP_044424067.1
• Coordinates: 4194498..4194917 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q48B52
• Locus tag: HQ966_RS19095
• Protein ID: WP_003381346.1
• Coordinates: 4194914..4195171 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ966_RS19060 (HQ966_18935)	4189867..4190622	+	756	WP_002552598.1	IS21-like element ISPsy14 family helper ATPase IstB	-
HQ966_RS19065 (HQ966_18940)	4190726..4191043	-	318	Protein_3771	transposase zinc-binding domain-containing protein	-
HQ966_RS19070 (HQ966_18945)	4191439..4192920	+	1482	WP_002556030.1	XopAG/AvrGf1 family type III secretion system effector	-
HQ966_RS19075 (HQ966_18950)	4193220..4193501	-	282	WP_002556029.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HQ966_RS19080 (HQ966_18955)	4193501..4193689	-	189	WP_002556028.1	hypothetical protein	-
HQ966_RS19085 (HQ966_18960)	4193866..4194457	-	592	Protein_3775	recombinase family protein	-
HQ966_RS19090 (HQ966_18965)	4194498..4194917	-	420	WP_044424067.1	type II toxin-antitoxin system VapC family toxin	Toxin
HQ966_RS19095 (HQ966_18970)	4194914..4195171	-	258	WP_003381346.1	Arc family DNA-binding protein	Antitoxin
HQ966_RS19100 (HQ966_18975)	4195694..4197193	+	1500	WP_122302115.1	IS21-like element ISPsy20 family transposase	-
HQ966_RS19105 (HQ966_18980)	4197186..4197989	+	804	WP_031598096.1	IS21-like element ISPsy20 family helper ATPase IstB	-
HQ966_RS19110 (HQ966_18985)	4198145..4198273	-	129	Protein_3780	transposase	-
HQ966_RS19115 (HQ966_18990)	4198314..4199873	-	1560	Protein_3781	HopW family type III effector protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15124.40 Da
• Isoelectric Point: 4.7010

>T209098 WP_044424067.1 NZ_CP078139:c4194917-4194498 [Pseudomonas savastanoi pv. savastanoi]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYVSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIAIANGFTVATRDTGPFEAAGLNVINPWEA

Nucleotide

• Length: 420 bp

>T209098 NZ_CP103834:1476273-1476533 [Actinobacillus genomosp. 1]
ATGAAACTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATTCAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACCGGAGCCGTTAAAATTCAATCTTTCGG
GCTTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCATGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9401.62 Da
• Isoelectric Point: 9.0330

>AT209098 WP_003381346.1 NZ_CP078139:c4195171-4194914 [Pseudomonas savastanoi pv. savastanoi]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Nucleotide

• Length: 258 bp

>AT209098 NZ_CP103834:1476025-1476276 [Actinobacillus genomosp. 1]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACAATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACGAGAAGCAAGGGCGGAAATTGCGTACTTATGTCATATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCACCTACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAT
TTAATCGAATGA

Structures

Antitoxin

• AlphaFold DB: Q48B52