Detailed information of TA system TA209094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 139250..139797
• Replicon: chromosome
• Accession: NZ_CP078139
• Organism: Pseudomonas savastanoi pv. savastanoi strain PVFi1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H1ZWV0
• Locus tag: HQ966_RS00650
• Protein ID: WP_015060661.1
• Coordinates: 139250..139525 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H1ZWV1
• Locus tag: HQ966_RS00655
• Protein ID: WP_015060662.1
• Coordinates: 139525..139797 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ966_RS00640 (HQ966_00625)	135622..137247	-	1626	WP_237901162.1	type III secretion system effector protein HopAB1	-
HQ966_RS00645 (HQ966_00630)	138054..139088	-	1035	WP_015060660.1	IS110-like element ISPsy16 family transposase	-
HQ966_RS00650 (HQ966_00635)	139250..139525	-	276	WP_015060661.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQ966_RS00655 (HQ966_00640)	139525..139797	-	273	WP_015060662.1	ribbon-helix-helix domain-containing protein	Antitoxin
HQ966_RS00660 (HQ966_00645)	140111..140452	+	342	Protein_132	transposase	-
HQ966_RS00665 (HQ966_00650)	140486..143002	-	2517	Protein_133	Tn3-like element ISPsy30 family transposase	-
HQ966_RS00670 (HQ966_00655)	143218..143490	+	273	Protein_134	Tn3 family transposase	-
HQ966_RS00675 (HQ966_00660)	143533..143757	-	225	WP_060410047.1	hypothetical protein	-
HQ966_RS00680 (HQ966_00665)	143795..143899	-	105	Protein_136	TraR/DksA family transcriptional regulator	-
HQ966_RS00685 (HQ966_00670)	144036..144173	+	138	WP_081084078.1	Tn3 family transposase	-
HQ966_RS00690 (HQ966_00675)	144269..144649	+	381	WP_044424089.1	CesT family type III secretion system chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	128542..157946	29404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10751.33 Da
• Isoelectric Point: 7.0126

>T209094 WP_015060661.1 NZ_CP078139:c139525-139250 [Pseudomonas savastanoi pv. savastanoi]
MELKWTSKALSDITRLYEFLALANQPAAARVVQQLTTAPTTLLTNPRIGERLEEFEPKDVRRIQIGQYEMRYEIADSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T209094 NZ_CP103833:c762714-762325 [Actinobacillus arthritidis]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGAGCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGTCGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGTCG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCTAGTGATAAAGCCTTTCAACTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10076.40 Da
• Isoelectric Point: 5.6994

>AT209094 WP_015060662.1 NZ_CP078139:c139797-139525 [Pseudomonas savastanoi pv. savastanoi]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT209094 NZ_CP103833:c762975-762715 [Actinobacillus arthritidis]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGATTTTAA

Structures

Toxin

• AlphaFold DB: A0A554FV80

Antitoxin

• AlphaFold DB: A0A3M4CJB9