Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2935901..2936738 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag KW063_RS14935 Protein ID WP_000227784.1
Coordinates 2936196..2936738 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag KW063_RS14930 Protein ID WP_001297137.1
Coordinates 2935901..2936212 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS14905 (2930921) 2930921..2931868 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
KW063_RS14910 (2931890) 2931890..2933881 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
KW063_RS14915 (2933871) 2933871..2934485 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
KW063_RS14920 (2934485) 2934485..2934814 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
KW063_RS14925 (2934826) 2934826..2935716 + 891 WP_000971336.1 heme o synthase -
KW063_RS14930 (2935901) 2935901..2936212 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
KW063_RS14935 (2936196) 2936196..2936738 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
KW063_RS14940 (2936794) 2936794..2937729 - 936 WP_001297127.1 tetratricopeptide repeat protein -
KW063_RS14945 (2938137) 2938137..2939501 + 1365 WP_001000978.1 MFS transporter -
KW063_RS14950 (2939629) 2939629..2940120 - 492 WP_001138904.1 nucleotide binding protein YajQ -
KW063_RS14955 (2940288) 2940288..2941199 + 912 WP_000705849.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T209089 WP_000227784.1 NZ_CP078137:2936196-2936738 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T209089 NZ_CP103831:502058-503380 [Actinobacillus equuli subsp. haemolyticus]
ATGGTAAAGCGTTTATGTGTGTTAATGAATGGGGAGTTAGTCGGTTTTTTTGAGCGTACTATGTCCGGCTCGCATTTATT
CCGTTATGCAGATGAGTGGTTTTTATCTCCAAATGTTAGACCGATTTCTCTTTCTATGCCATTAAGAAGGGAAGTTTATC
AAGGTAAAGAAGTGATTAATTTTTTTGATAACTTATTACCAGATCACCCTCAGGTTAGAGCTAGAATAGCGGCTCGATTT
CAGGCTGATACACAACAACCTTTTGAATTATTAGCTAAGATTGGGCGAGACGCAACCGGAGCAATAACCTTACTAGAGGA
AAACACCGAAATTCCCAATCATAAAGTTATTCATGCAACGGAATTAAACGAAAAGGAGCTTCATTCGTTATTATTAGGTT
ATCGGCAAAATGCACCGCTTGGTATGTTAGATGAAGCTCAAGATTTTCGAATTTCTATCGCAGGGGCTCAAGAGAAGACA
GCGTTATTATGGTATAACGAACGCTGGCAATTACCTCACGGATTAACTCCAACGACACATATTATTAAATTACCGATAGG
TAAAATACAAACACCTAAATATCAATTAGATCTGTCTCAAAGTGTAGATAACGAATTGATTTGTTTACGTTTATGCGCAG
CGTTTGGTTTACCGAGCGCAAATGTAAATTTATTAGAGGTCGGGGATGTACGAGCATTAGTGGTAGAACGATTTGATCGT
AAATTACATTCTTCAAATCAATATTTTTTACGCCTACCTTATGAAGATTTTTGCCAAGTATTCGGTTTGGCTCCAACTCA
AAAATATGAGAGTGATGGTGGAGTCGGGATTCGCCACATTATGGATAAACTTTTGTATTCCAATCAAATTTCAGATCGAA
CGCAATTCATGCGGACTCAGTTTTTATTTTGGGTGCTTGGGGCGATTGATGGGCATGCGAAAAACTTTTCTATTGCATTG
AAGTCGCAGAGTCGTTATCACTTATCTCCAATTTATGATGTTATATCGGCTTATCCAATGTTGGGAGGTAAAGGGTGGCA
TCAGAGTGATTTGCAACTTGCGATGAGTCTGTGTAGTACCCAAAAGGGTAAAAAGTGGAGTTGGCAAAAAATACGACCGG
TACATTATCTGTCTACCGCTAAATCTGTCGGATTTAATCAGTTAGAAATGAAACATATTATTGAAGAGATTTCGGATACG
GCATTAAGAAAGTTAGATTTTGTAGCTGATAGGCTGCCAATAAATATTAATGAACAAGTCAGAGATCAAATTTTTAATGG
CGTTAAAAAACAGATTGGTAAAATCAAATTAGATAACATTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT209089 WP_001297137.1 NZ_CP078137:2935901-2936212 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT209089 NZ_CP103831:501822-502064 [Actinobacillus equuli subsp. haemolyticus]
ATGCTAATCAATGATCCTAATAGTTTAGCCGTTTATTTACGCGATATTCGTAAGCAACAGAAACAGTCACAAAAAATGAT
TGCCCAACGAGTCGGCTTACGACAAGCAACGATCTCTACGTTTGAAAGTCAGCCGGATAAAGTTACAATAGAAACACTAT
TTAAAATAGCCCATTCTCTTGGTTTGGAAATCCATTTAGAAGAAAAACATAAGAATATGTTTAATGGGAGCGATGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References