Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2901824..2902442 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KW063_RS14765 Protein ID WP_001291435.1
Coordinates 2902224..2902442 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KW063_RS14760 Protein ID WP_000344800.1
Coordinates 2901824..2902198 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS14750 (2896913) 2896913..2898106 + 1194 WP_069907026.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KW063_RS14755 (2898129) 2898129..2901278 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KW063_RS14760 (2901824) 2901824..2902198 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KW063_RS14765 (2902224) 2902224..2902442 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KW063_RS14770 (2902613) 2902613..2903164 + 552 WP_000102564.1 maltose O-acetyltransferase -
KW063_RS14775 (2903280) 2903280..2903750 + 471 WP_000136192.1 YlaC family protein -
KW063_RS14780 (2903914) 2903914..2905464 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KW063_RS14785 (2905506) 2905506..2905859 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KW063_RS14795 (2906238) 2906238..2906549 + 312 WP_000409911.1 MGMT family protein -
KW063_RS14800 (2906580) 2906580..2907152 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209088 WP_001291435.1 NZ_CP078137:2902224-2902442 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T209088 NZ_CP103831:67512-67934 [Actinobacillus equuli subsp. haemolyticus]
ATGTATTTATTAGATACCAATATTGTTTCCGAATTACGTAAAATGGAAAGTGGAAAAGCCGATGTTAATGTGGTGAAATG
GTTTCAACAAATCAATTTAACCGATGCTTATTTAAGTGTTATCACGCTCTTTGAAATTAAGTTAGGTATTTTGCAACTTA
AACGCAAAGATATTCAACAAGCTAAACTTTTACAAAGTTGGTTTGAACAAAAACTCCTGCCTAATTTTGAAAATAGAATT
TTACCGCTTGATTCAAAAGTGATGATGACTTGTGCAGAGTTTCATATTCCGGATAAAAAGCCATTAAATGATTCTTATAT
AGCCGCTACGGCTAAAGCACATAGATTTAAAATTGTGAGCAGAAATGTGAAAGATTTTAAGGGATGTGGAGTGGAGGTTT
TAAATCCATTTGCTGAGTCTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT209088 WP_000344800.1 NZ_CP078137:2901824-2902198 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT209088 NZ_CP103831:67225-67512 [Actinobacillus equuli subsp. haemolyticus]
ATGGCGGTAATCAGTTCTAAAGAATTTAACCAACGAGCCAGTGCGGTATTAAAAATTGCCGAAACCGAGCCGGTATATAT
TACTAAGTGGGGGAAAGTGGTTAGTGTATTGGCAAGTTATGCTCATTATCAAGCTCAAACCAAATCGCAAAGTTTAGCGG
AGGCTTTTAGCGGTACAGCTCTGACAGAAATATTAAGCGAGGAAGCGGAACAGCAATTTGAACGAACTTTGCAGCAAGTA
CGCCAACAAAGCAAAATGAGTGTGCCTGATTTATCGGAGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References