Detailed information of TA system TA209087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1853236..1853874
• Replicon: chromosome
• Accession: NZ_CP078137
• Organism: Escherichia coli strain Sx181-0-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KW063_RS09655
• Protein ID: WP_000813794.1
• Coordinates: 1853698..1853874 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KW063_RS09650
• Protein ID: WP_001270285.1
• Coordinates: 1853236..1853652 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW063_RS09630 (1848388)	1848388..1849329	-	942	WP_001251315.1	ABC transporter permease	-
KW063_RS09635 (1849330)	1849330..1850343	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
KW063_RS09640 (1850361)	1850361..1851506	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
KW063_RS09645 (1851751)	1851751..1853157	-	1407	WP_000760594.1	PLP-dependent aminotransferase family protein	-
KW063_RS09650 (1853236)	1853236..1853652	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KW063_RS09655 (1853698)	1853698..1853874	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KW063_RS09660 (1854096)	1854096..1854326	+	231	WP_000494244.1	YncJ family protein	-
KW063_RS09665 (1854418)	1854418..1856379	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KW063_RS09670 (1856452)	1856452..1856988	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KW063_RS09675 (1857041)	1857041..1858255	+	1215	WP_071601296.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1858295..1859443	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T209087 WP_000813794.1 NZ_CP078137:c1853874-1853698 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T209087 NZ_CP103830:2336614-2336985 [Actinobacillus equuli subsp. equuli]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT209087 WP_001270285.1 NZ_CP078137:c1853652-1853236 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT209087 NZ_CP103830:2336966-2337256 [Actinobacillus equuli subsp. equuli]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTTCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTAGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Structures

Toxin

• PDB: 6HPB