Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 805963..806588 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KW063_RS04715 Protein ID WP_000911329.1
Coordinates 806190..806588 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KW063_RS04710 Protein ID WP_000450524.1
Coordinates 805963..806190 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS04685 (801765) 801765..802235 - 471 WP_218335287.1 thioredoxin-dependent thiol peroxidase -
KW063_RS04690 (802235) 802235..802807 - 573 WP_000176191.1 glycine cleavage system transcriptional repressor -
KW063_RS04695 (802953) 802953..803831 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KW063_RS04700 (803848) 803848..804882 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KW063_RS04705 (805095) 805095..805808 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KW063_RS04710 (805963) 805963..806190 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KW063_RS04715 (806190) 806190..806588 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KW063_RS04720 (806735) 806735..807598 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KW063_RS04725 (807613) 807613..809628 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
KW063_RS04730 (809702) 809702..810400 + 699 WP_000679823.1 esterase -
KW063_RS04735 (810510) 810510..810710 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T209076 WP_000911329.1 NZ_CP078137:806190-806588 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T209076 NZ_CP103826:1975949-1976290 [Actinobacillus equuli subsp. haemolyticus]
ATGAAGTTAGTTTTTGTAGAATTACCACCTTTTGATAAATTTCGTTATGAAAATTTAACAGATGATGATTATCGTGAATT
TCAAGAATATTTGATGAATAATCCCGAAGTCGGAGATATTGTTCAAGGCGTAAACGGTTTACGAAAAATTCGTATATCAA
CCCAAGCAAAGGGGAAGAAAGGTGGTGCCAGAGTGATTTACTACTATTATGTTAGAGGATCACAAATTTGGTTATTTACA
GGGTATAACAAATCAAGAAAAATTGATTTGAATTCAATGGAAAGAAAAGCATTTTCTAAAGTATTAGAACATTTAAAAAA
TATTGCGGATAGGGAGCTATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT209076 WP_000450524.1 NZ_CP078137:805963-806190 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT209076 NZ_CP103826:1976291-1976599 [Actinobacillus equuli subsp. haemolyticus]
ATGGGACGTTTATTTGATGATCTGATGGAAGGGGCGGAAGCCTTAGAACAATACCTTCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCGATAGAACTTAGCCCGTCCGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCACATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAACAAGGTAAAACCAAACCGAATCCA
CAAGCCACTTTGTTATTACGAATGGTAGACAAATCTCCGCAATTATTAGAACAAATGGCACAACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References