Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 447403..447986 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KW063_RS02890 Protein ID WP_000254745.1
Coordinates 447651..447986 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KW063_RS02885 Protein ID WP_000581937.1
Coordinates 447403..447651 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS02875 (443742) 443742..445043 + 1302 WP_032197155.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KW063_RS02880 (445091) 445091..447325 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KW063_RS02885 (447403) 447403..447651 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KW063_RS02890 (447651) 447651..447986 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KW063_RS02895 (448057) 448057..448848 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
KW063_RS02900 (449076) 449076..450713 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KW063_RS02905 (450801) 450801..452099 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T209074 WP_000254745.1 NZ_CP078137:447651-447986 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T209074 NZ_CP103826:821932-822114 [Actinobacillus equuli subsp. haemolyticus]
GTGACAAGTGCGGAGCTCATCAAATTAATTGAAAGTGACGGTTGGTATAGAGTCAATATAGCGGGGAGTCATCATCAGTT
TAAACACCCCACAAAAAAAGGGCGAGTAACCATACCTCACCCTAAAAAGGACCTTAATATCAAAACACAAAATTCAATAT
TAAAACAAGCGGGGCTGAAATAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT209074 WP_000581937.1 NZ_CP078137:447403-447651 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT209074 NZ_CP103826:822138-822545 [Actinobacillus equuli subsp. haemolyticus]
ATGTTATATCCGATTGCGATTGAACTTGGCGATGAGAAACACGCCTATGGTGTAGTTGTACCTGATGTACCCGGTTGTTT
TTCTGCCGGTGATACGATTGAAGAAGCATTCCAAAATGCAAAAGAAGCGATTCCGTTTCATATTGAAGGGATGTTAGAAG
ACGGGGAGGAGATTCCGCAACCGACAAGCATTGATACACACCGTAATAATCCTGACTATGAAGGCTTTATGTGGTCTGTG
GTTGATGTAGATCTCACTCACTTAATGGGCAAATCAGAAAAACTTAATATCACTTTGCCAAGCCTCTTAATTAAACGTAT
TGATGAGTTTGTCGCACGTCATCCGGATTATAAAAACCGGAGTAACTTTTTAGCGAAAGTGGCGACAGACCGCTTATTAA
GTGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References