Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 311077..311731 Replicon chromosome
Accession NZ_CP078137
Organism Escherichia coli strain Sx181-0-1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KW063_RS02265 Protein ID WP_000244781.1
Coordinates 311324..311731 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KW063_RS02260 Protein ID WP_000354046.1
Coordinates 311077..311343 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW063_RS02235 (306246) 306246..306989 + 744 WP_000951951.1 SDR family oxidoreductase -
KW063_RS02240 (307046) 307046..308479 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KW063_RS02245 (308524) 308524..308835 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KW063_RS02250 (308999) 308999..309658 + 660 WP_000250274.1 hemolysin III family protein -
KW063_RS02255 (309854) 309854..310834 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
KW063_RS02260 (311077) 311077..311343 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KW063_RS02265 (311324) 311324..311731 + 408 WP_000244781.1 protein YgfX Toxin
KW063_RS02270 (311771) 311771..312292 - 522 WP_001055874.1 flavodoxin FldB -
KW063_RS02275 (312404) 312404..313300 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KW063_RS02280 (313325) 313325..314035 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KW063_RS02285 (314041) 314041..315774 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T209073 WP_000244781.1 NZ_CP078137:311324-311731 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T209073 NZ_CP103825:2310263-2310634 [Actinobacillus equuli subsp. haemolyticus]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT209073 WP_000354046.1 NZ_CP078137:311077-311343 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT209073 NZ_CP103825:2310615-2310905 [Actinobacillus equuli subsp. haemolyticus]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAGCGAGAAGTATTTAACGAGTTAGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References