Detailed information of TA system TA209069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 28226..28851
• Replicon: plasmid unnamed1
• Accession: NZ_CP078136
• Organism: Escherichia coli strain Sx181-0-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KW063_RS00180
• Protein ID: WP_218335155.1
• Coordinates: 28226..28624 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: KW063_RS00185
• Protein ID: WP_000450532.1
• Coordinates: 28624..28851 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW063_RS00165 (24930)	24930..25439	+	510	WP_218335151.1	conjugal transfer entry exclusion protein TraS	-
KW063_RS00170 (25606)	25606..25839	+	234	Protein_34	complement resistance protein TraT	-
KW063_RS00175 (26064)	26064..28217	+	2154	WP_218335153.1	type IV conjugative transfer system coupling protein TraD	-
KW063_RS00180 (28226)	28226..28624	-	399	WP_218335155.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KW063_RS00185 (28624)	28624..28851	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	paa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / cofA / faeC	1..136082	136082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14950.28 Da
• Isoelectric Point: 9.8204

>T209069 WP_218335155.1 NZ_CP078136:c28624-28226 [Escherichia coli]
MLKFMLDTNICIFTIKNKPARVRDRFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLHYDAAAATHT
GQIRAELSRQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T209069 NZ_CP103824:c428104-427742 [Actinobacillus equuli subsp. haemolyticus]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGACATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTTGTAATGGGAAAACTTTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT209069 WP_000450532.1 NZ_CP078136:c28851-28624 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT209069 NZ_CP103824:c427758-427450 [Actinobacillus equuli subsp. haemolyticus]
ATGGGAAAACTTTTTGATGATTTAATGGAAGGGCTTGATGCTTATGAAAAACATATCCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCAGTAGAACTTAGCCCGTCTGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAGCAAGGCAAAACTAAACCGAATCCA
CAAGCTACTTTGTTATTACGAATGGTGGACAAATCTCCGCAATTATTAGAACAAATGGCACAGCTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5