Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pemIK/PRK09907-MazE |
| Location | 25497..26098 | Replicon | plasmid pGR1187-2 |
| Accession | NZ_CP078134 | ||
| Organism | Lactiplantibacillus plantarum subsp. plantarum strain GR1187 | ||
Toxin (Protein)
| Gene name | pemK | Uniprot ID | Q684P1 |
| Locus tag | KVG02_RS15450 | Protein ID | WP_001748110.1 |
| Coordinates | 25497..25841 (-) | Length | 115 a.a. |
Antitoxin (Protein)
| Gene name | pemI | Uniprot ID | A0A806J926 |
| Locus tag | KVG02_RS15455 | Protein ID | WP_001748109.1 |
| Coordinates | 25835..26098 (-) | Length | 88 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KVG02_RS15415 (KVG02_15415) | 20886..21293 | + | 408 | WP_046811104.1 | hypothetical protein | - |
| KVG02_RS15420 (KVG02_15420) | 21409..21819 | + | 411 | WP_046811105.1 | hypothetical protein | - |
| KVG02_RS15425 (KVG02_15425) | 21942..22244 | - | 303 | WP_005917927.1 | type II toxin-antitoxin system YafQ family toxin | - |
| KVG02_RS15430 (KVG02_15430) | 22234..22512 | - | 279 | WP_003625288.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
| KVG02_RS15435 (KVG02_15435) | 22876..23064 | - | 189 | Protein_24 | IS3 family transposase | - |
| KVG02_RS15440 (KVG02_15440) | 23190..24242 | + | 1053 | WP_046811107.1 | ABC transporter permease | - |
| KVG02_RS15445 (KVG02_15445) | 24247..24927 | + | 681 | WP_046811108.1 | ABC transporter ATP-binding protein | - |
| KVG02_RS15450 (KVG02_15450) | 25497..25841 | - | 345 | WP_001748110.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| KVG02_RS15455 (KVG02_15455) | 25835..26098 | - | 264 | WP_001748109.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| KVG02_RS15460 (KVG02_15460) | 26184..26771 | + | 588 | WP_046811109.1 | site-specific integrase | - |
| KVG02_RS15465 (KVG02_15465) | 27145..27339 | + | 195 | WP_046811110.1 | hypothetical protein | - |
| KVG02_RS15470 (KVG02_15470) | 27586..28366 | + | 781 | Protein_31 | IS5 family transposase | - |
| KVG02_RS15475 (KVG02_15475) | 29182..29529 | - | 348 | WP_256353672.1 | hypothetical protein | - |
| KVG02_RS15480 (KVG02_15480) | 29754..30683 | + | 930 | WP_218460883.1 | IS30-like element ISLpl1 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..30697 | 30697 | |
| - | flank | IS/Tn | - | - | 29754..30683 | 929 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 115 a.a. Molecular weight: 13041.89 Da Isoelectric Point: 6.4782
>T209068 WP_001748110.1 NZ_CP078134:c25841-25497 [Lactiplantibacillus plantarum subsp. plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK
Download Length: 345 bp
>T209068 NZ_CP103824:c90332-89961 [Actinobacillus equuli subsp. haemolyticus]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTCGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTCGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG
Antitoxin
Download Length: 88 a.a. Molecular weight: 10141.47 Da Isoelectric Point: 5.7117
>AT209068 WP_001748109.1 NZ_CP078134:c26098-25835 [Lactiplantibacillus plantarum subsp. plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW
Download Length: 264 bp
>AT209068 NZ_CP103824:c89980-89690 [Actinobacillus equuli subsp. haemolyticus]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0R2K1X3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A806J926 |