Detailed information of TA system TA209065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 62259..63169
• Replicon: plasmid pKP169-P1
• Accession: NZ_CP078124
• Organism: Klebsiella pneumoniae strain KPC-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: KU889_RS28865
• Protein ID: WP_004026354.1
• Coordinates: 62699..63169 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: KU889_RS28860
• Protein ID: WP_004026357.1
• Coordinates: 62259..62702 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU889_RS28830	57294..57695	+	402	WP_214588034.1	LuxR C-terminal-related transcriptional regulator	-
KU889_RS28835	57768..58670	+	903	WP_004213613.1	DMT family inner membrane transporter PEG344	-
KU889_RS28840	58813..59034	-	222	WP_004213615.1	hypothetical protein	-
KU889_RS28845	59096..60403	-	1308	Protein_71	FepA family TonB-dependent siderophore receptor	-
KU889_RS28850	60469..61449	+	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
KU889_RS28855	61655..62158	+	504	Protein_73	DUF4113 domain-containing protein	-
KU889_RS28860	62259..62702	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
KU889_RS28865	62699..63169	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
KU889_RS28870	63318..64015	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
KU889_RS28875	65401..66051	-	651	WP_068893702.1	DUF1173 family protein	-
KU889_RS30935	66084..66350	-	267	WP_223175074.1	DUF1173 family protein	-
KU889_RS28880	66528..67022	+	495	WP_004212794.1	thermonuclease family protein	-
KU889_RS28885	67359..67757	+	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN	1..221577	221577
	-	inside	IScluster/Tn	-	rmpA / iroN	47073..69208	22135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T209065 WP_004026354.1 NZ_CP078124:62699-63169 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T209065 NZ_CP103821:2314239-2314610 [Actinobacillus equuli subsp. equuli]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT209065 WP_004026357.1 NZ_CP078124:62259-62702 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT209065 NZ_CP103821:2314591-2314881 [Actinobacillus equuli subsp. equuli]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9