Detailed information of TA system TA209064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 18835..19505
• Replicon: plasmid pKP169-P1
• Accession: NZ_CP078124
• Organism: Klebsiella pneumoniae strain KPC-2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KU889_RS28610
• Protein ID: WP_004213072.1
• Coordinates: 19062..19505 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KU889_RS28605
• Protein ID: WP_004213073.1
• Coordinates: 18835..19065 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU889_RS28585	14766..14933	-	168	Protein_17	IS1 family transposase	-
KU889_RS28590	15237..16166	+	930	WP_004213558.1	Rpn family recombination-promoting nuclease/putative transposase	-
KU889_RS28595	16311..17090	-	780	WP_004213560.1	site-specific integrase	-
KU889_RS28600	17087..17908	-	822	WP_004213562.1	hypothetical protein	-
KU889_RS28605	18835..19065	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KU889_RS28610	19062..19505	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KU889_RS30915	19654..19905	+	252	WP_186987481.1	hypothetical protein	-
KU889_RS28620	19928..20232	-	305	Protein_24	transposase	-
KU889_RS28625	20649..21284	+	636	Protein_25	mucoid phenotype regulator RmpA2	-
KU889_RS28630	21802..22205	-	404	Protein_26	GAF domain-containing protein	-
KU889_RS28635	22296..23216	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
KU889_RS28640	23265..23756	-	492	WP_011251327.1	DM13 domain-containing protein	-
KU889_RS28645	23819..24094	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN	1..221577	221577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T209064 WP_004213072.1 NZ_CP078124:19062-19505 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T209064 NZ_CP103821:2021180-2021542 [Actinobacillus equuli subsp. equuli]
GTGAAGTTAGTTTTTGTAGAATTACCACCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGACATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTTGTAATGGGAAAACTTTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT209064 WP_004213073.1 NZ_CP078124:18835-19065 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT209064 NZ_CP103821:2021526-2021834 [Actinobacillus equuli subsp. equuli]
ATGGGAAAACTTTTTGATGATTTAATGGAAGGGCTTGATGCTTATGAAAAACATATCCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCAGTAGAACTTAGCCCGTCTGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAGCAAGGCAAAACTAAACCGAATCCA
CAAGCTACTTTGTTATTACGAATGGTGGACAAATCTCCGCAATTATTAGAACAAATGGCACAGCTCTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620