Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33670..34313 Replicon plasmid pKP169-P2
Accession NZ_CP078123
Organism Klebsiella pneumoniae strain KPC-2

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KU889_RS27670 Protein ID WP_001044770.1
Coordinates 33897..34313 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KU889_RS27665 Protein ID WP_001261282.1
Coordinates 33670..33900 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU889_RS27630 (28893) 28893..29672 - 780 WP_013214009.1 site-specific integrase -
KU889_RS27635 (29856) 29856..30860 - 1005 WP_011977814.1 hypothetical protein -
KU889_RS27640 (30890) 30890..31093 - 204 WP_011977813.1 hypothetical protein -
KU889_RS27645 (31139) 31139..31660 - 522 WP_013214008.1 hypothetical protein -
KU889_RS27650 (31718) 31718..32110 - 393 WP_011977811.1 hypothetical protein -
KU889_RS27655 (32147) 32147..33091 - 945 WP_011977810.1 hypothetical protein -
KU889_RS27660 (33252) 33252..33713 - 462 WP_014343465.1 hypothetical protein -
KU889_RS27665 (33670) 33670..33900 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KU889_RS27670 (33897) 33897..34313 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KU889_RS27675 (34387) 34387..35949 + 1563 WP_004206609.1 AAA family ATPase -
KU889_RS27680 (35934) 35934..36956 + 1023 WP_000361404.1 helicase UvrD -
KU889_RS27685 (37212) 37212..37909 + 698 WP_223174630.1 IS1 family transposase -
KU889_RS27690 (37938) 37938..38210 + 273 Protein_50 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 - 1..157394 157394


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T209056 WP_001044770.1 NZ_CP078123:33897-34313 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T209056 NZ_CP103818:508727-510049 [Actinobacillus equuli subsp. haemolyticus]
ATGGTAAAGCGTTTATGTGTGTTAATGAATGGGGAGTTAGTCGGTTTTTTTGAGCGTACTATGTCCGGCTCGCATTTATT
CCGTTATGCAGATGAGTGGTTTTTATCTCCAAATGTTAGACCGATTTCTCTTTCTATGCCATTAAGAAGGGAAGTTTATC
AAGGTAAAGAAGTGATTAATTTTTTTGATAACTTATTACCAGATCACCCTCAGGTTAGAGCTAGAATAGCGGCTCGATTT
CAGGCTGATACACAACAACCTTTTGAATTATTAGCTAAGATTGGGCGAGACGCAACCGGAGCAATAACCTTACTAGAGGA
AAACACCAAAATTCCCAATCATAAAGTTATTCATGCAACGGAATTAAACGAAAAGGAGCTTCATTCGTTATTATTAGGTT
ATCGGCAAAATGCACCGCTTGGTATGTTAGATGAAGCTCAAGATTTTCGAATTTCTATCGCAGGGGCTCAAGAGAAGACA
GCGTTATTATGGTATAACGAACGCTGGCAATTACCTCACGGATTAACTCCAACGACACATATTATTAAATTACCGATAGG
CAAAATACAAACACCTAAATATCAATTAGATCTGTCTCAAAGTGTAGATAACGAATTGATTTGTTTACGTTTATGCGCAG
CGTTTGGTTTACCGAGCGCAAATGTAAATTTATTAGAGGTCGGGGATGTACGAGCATTAGTGGTAGAACGATTTGATCGT
AAATTACATTCTTCAAATCAATATTTTTTACGCCTACCTTATGAAGATTTTTGCCAAGTATTCGGTTTGGCTCCAACTCA
AAAATATGAGAGTGATGGTGGAGTCGGGATTCGCCACATTATGGATAAACTTTTGTATTCCAATCAAATTTCAGATCGAA
CGCAATTCATGCGGACTCAGTTTTTATTTTGGGTGCTTGGGGCGATTGATGGGCATGCGAAAAACTTTTCTATTGCATTG
AAGTCGCAGAGTCGTTATCACTTATCTCCAATTTATGATGTTATATCGGCTTATCCAATGTTGGGAGGTAAAGGGTGGCA
TCAGAGTGATTTGCAACTTGCGATGAGTCTGTGTAGTACCCAAAAGGGTAAAAAGTGGAGTTGGCAAAAAATACGACCGG
TACATTATCTGTCTACCGCTAAATCTGTCGGATTTAATCAGTTAGAAATGAAACATATTATTGAAGAGATTTCGGATACG
GCATTAAGAAAGTTAGATTTTGTAGCTGATAGGCTGCCAATAAATATTAATGAACAAGTCAGAGATCAAATTTTTAATGG
CGTTAAAAAACAGATTGGTAAAATCAAATTAGATAACATTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT209056 WP_001261282.1 NZ_CP078123:33670-33900 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT209056 NZ_CP103818:508491-508733 [Actinobacillus equuli subsp. haemolyticus]
ATGCTAATCAATGATCCTAATAGTTTAGCCGTTTATTTACGCGATATTCGTAAGCAACAGAAACAGTCACAAAAAATGAT
TGCCCAACGAGTCGGCTTACGACAAGCAACGATCTCTACGTTTGAAAGTCAGCCGGATAAAGTTACAATAGAAACACTAT
TTAAAATAGCCCATTCTCTTGGTTTGGAAATCCATTTAGAAGAAAAACATAAGAATATGTTTAATGGGAGCGATGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References