Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5448431..5449056 Replicon chromosome
Accession NZ_CP078122
Organism Klebsiella pneumoniae strain KPC-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KU889_RS27085 Protein ID WP_002882817.1
Coordinates 5448431..5448814 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KU889_RS27090 Protein ID WP_004150355.1
Coordinates 5448814..5449056 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU889_RS27070 5445797..5446699 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KU889_RS27075 5446696..5447331 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KU889_RS27080 5447328..5448257 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KU889_RS27085 5448431..5448814 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KU889_RS27090 5448814..5449056 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KU889_RS27095 5449261..5450178 + 918 WP_002882812.1 alpha/beta hydrolase -
KU889_RS27100 5450192..5451133 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KU889_RS27105 5451178..5451615 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KU889_RS27110 5451612..5452472 - 861 WP_002882807.1 virulence factor BrkB family protein -
KU889_RS27115 5452466..5453065 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T209055 WP_002882817.1 NZ_CP078122:c5448814-5448431 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T209055 NZ_CP103817:2281575-2281946 [Actinobacillus equuli subsp. equuli]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACAATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT209055 WP_004150355.1 NZ_CP078122:c5449056-5448814 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT209055 NZ_CP103817:2281927-2282217 [Actinobacillus equuli subsp. equuli]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAACGAGAGGTGTTTAATGAGTTGGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References