Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4864903..4865713 Replicon chromosome
Accession NZ_CP078122
Organism Klebsiella pneumoniae strain KPC-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KU889_RS24290 Protein ID WP_002887280.1
Coordinates 4864903..4865436 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KU889_RS24295 Protein ID WP_002887278.1
Coordinates 4865447..4865713 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU889_RS24285 4863734..4864855 + 1122 WP_002887282.1 cupin domain-containing protein -
KU889_RS24290 4864903..4865436 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KU889_RS24295 4865447..4865713 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KU889_RS24300 4865816..4867249 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KU889_RS24305 4867239..4867922 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KU889_RS24310 4868094..4869479 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KU889_RS24315 4869497..4869841 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T209052 WP_002887280.1 NZ_CP078122:c4865436-4864903 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T209052 NZ_CP103816:1985497-1985859 [Actinobacillus equuli subsp. equuli]
GTGAAGTTAGTTTTTGTAGAATTACCTCCTTTTGAGCGATATAGACAAAAGCACCTTAGTGATGAAGAATATAGACATTT
TCAGAATGAGCTATTAGAAAATCCTAATAAAGGAGATTTAATCCAAGGTACGAACGGCTTACGCAAAATTAGAATTGCAG
ATATGCAACGAAATAAAGGTAAGAGAGGTGGAGCAAGGGTAATTTATTATCATATTGTGAATTATTCAAAAATTTTGTTG
GTTACCGCATATGGTAAAGATGAACAATCAGATTTATCTAATACGGAACGCAATATGTTAGCGAATAAAGTGAAGCGTAT
TGTTGAATTAGAGATAAGGAGTTGTAATGGGAAAACTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT209052 WP_002887278.1 NZ_CP078122:c4865713-4865447 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT209052 NZ_CP103816:1985843-1986151 [Actinobacillus equuli subsp. equuli]
ATGGGAAAACTTTTTGATGATTTAATGGAAGGGCTTGATGCTTATGAAAAACATATCCAAGGCAAAATCACCCTGCGAAC
CACGGTATTAGAAAAGCCTGAACCAGTAGAACTTAGTCCGTCTGAAGTTAAATCGATTCGAGAAAAATTAAATCTGTCGC
AAGCGGTTTTTGCGCATAAATTGCATACTTCCGTGCGGACTTATCAAGGGTGGGAGCAAGGCAAAACTAAACCGAATCCA
CAAGCTACTTTGTTATTACGAATGGTGGACAAATCTCCGCAATTATTAGAACAAATGGCACAGCTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References