Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4251013..4251632 Replicon chromosome
Accession NZ_CP078122
Organism Klebsiella pneumoniae strain KPC-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU889_RS21340 Protein ID WP_002892050.1
Coordinates 4251414..4251632 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KU889_RS21335 Protein ID WP_002892066.1
Coordinates 4251013..4251387 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU889_RS21325 4246165..4247358 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KU889_RS21330 4247381..4250527 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
KU889_RS21335 4251013..4251387 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KU889_RS21340 4251414..4251632 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KU889_RS21345 4251791..4252357 + 567 WP_002892030.1 maltose O-acetyltransferase -
KU889_RS21350 4252329..4252469 - 141 WP_004147370.1 hypothetical protein -
KU889_RS21355 4252490..4252960 + 471 WP_002892026.1 YlaC family protein -
KU889_RS21360 4252935..4254386 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KU889_RS21365 4254487..4255185 + 699 WP_002892021.1 GNAT family protein -
KU889_RS21370 4255182..4255322 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU889_RS21375 4255322..4255585 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T209051 WP_002892050.1 NZ_CP078122:4251414-4251632 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T209051 NZ_CP103815:2350151-2350522 [Actinobacillus equuli subsp. haemolyticus]
ATGTATGAAATAGTCTTTTATCGAGACCGACGTGGGCGAGAACCCGTCAAGGAATTCCTGCAGGAATTTATTAATGAACA
ACAAGACGAAAATCGAGAGCGTTTGCACAAAATTAGTCATCATTTGACGATTTTACATTTGCACGGCACTCGAGCAGGCG
AAAGCTATATTAAGCATTTAGAAGATCGTATTTGGCAGTTACGTCCAATTAGCGATTGTTTGCTCTTCGCCGGTATTGTG
CGAGGTCAGTTTGTATTGTTGCATCATTTTGCCAAAAGCAGTAGTCGGCTTCCTAAGCGTGAGCTTGAGCGAGCAAAAAG
TCGTTTGGCAGATCTACAGGAGAGGATAAAAGATGAGCCCCATTGGTTCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT209051 WP_002892066.1 NZ_CP078122:4251013-4251387 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT209051 NZ_CP103815:2350503-2350793 [Actinobacillus equuli subsp. haemolyticus]
ATGAGCCCCATTGGTTCTAGTTGGACATCATTTGAGCGAGAAGTATTTAACGAGTTAGAAATGGAAGAAAACCATTTCCG
CGTTAAATTAATTCTTGAAATCATTGAAACCCGACAAGCACTAGGCATTTCACAGCGTAAATTAGAAAAACTAAGCGGCG
TTAAACAATCTATGATTGCTCGCATTGAAAAGGGAAGTTCCAATCCTTCTCTTGGTACGTTACTGAAGTTACTGGTGCCT
TTGGGGAAAACATTACAAATTGTCTCTTTAGACGAAACTCATTCTAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References