Detailed information of TA system TA209009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 13079..13891
• Replicon: plasmid pH322_1
• Accession: NZ_CP078111
• Organism: Cronobacter sakazakii strain MOD1-H322

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: BTK77_RS20775
• Protein ID: WP_192575777.1
• Coordinates: 13373..13891 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A853H7H9
• Locus tag: BTK77_RS20770
• Protein ID: WP_029463984.1
• Coordinates: 13079..13348 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTK77_RS20750 (BTK77_020745)	9350..10075	-	726	WP_029463882.1	hypothetical protein	-
BTK77_RS20755 (BTK77_020750)	10283..11446	-	1164	WP_029590324.1	hypothetical protein	-
BTK77_RS20760 (BTK77_020755)	11652..12005	-	354	WP_161576472.1	hypothetical protein	-
BTK77_RS20765 (BTK77_020760)	12011..12676	-	666	WP_029590320.1	division plane positioning ATPase MipZ	-
BTK77_RS20770 (BTK77_020765)	13079..13348	+	270	WP_029463984.1	DUF1778 domain-containing protein	Antitoxin
BTK77_RS20775 (BTK77_020770)	13373..13891	+	519	WP_192575777.1	GNAT family N-acetyltransferase	Toxin
BTK77_RS20780 (BTK77_020775)	14075..14326	-	252	WP_032803499.1	hypothetical protein	-
BTK77_RS20785 (BTK77_020780)	14328..15020	-	693	WP_029590318.1	membrane protein	-
BTK77_RS20790 (BTK77_020785)	15032..15355	-	324	WP_029463980.1	hypothetical protein	-
BTK77_RS21995	15445..17973	-	2529	WP_235608080.1	tail fiber domain-containing protein	-
BTK77_RS20800 (BTK77_020795)	18045..18692	-	648	WP_029590316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..100741	100741

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 19245.83 Da
• Isoelectric Point: 6.9550

>T209009 WP_192575777.1 NZ_CP078111:13373-13891 [Cronobacter sakazakii]
MFSEEAVYDFSDFDCGEASLNEFLKNRLAQQHSGRILRGYLLLTKDAIPKVKGFYTLSGSCFARQTLPSNTQQRKIPYSD
APSVTLGRLAIDKSIQRQGKGEELVVDAMKVVYQASRAVGIYALFVDAKNPAARQFYQNLGFIPLKGENANSLFYPTKSI
EALFEEKPSEDE

Nucleotide

• Length: 519 bp

>T209009 NZ_CP103791:310763-311131 [Salmonella enterica subsp. enterica serovar Infantis]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTTGGCATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10153.64 Da
• Isoelectric Point: 7.0160

>AT209009 WP_029463984.1 NZ_CP078111:13079-13348 [Cronobacter sakazakii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMNALSNPPAPNERLKR
AAKRLQNME

Nucleotide

• Length: 270 bp

>AT209009 NZ_CP103791:310545-310766 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGATCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A853H7H9