Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 3704168..3704753 Replicon chromosome
Accession NZ_CP078110
Organism Cronobacter sakazakii strain MOD1-H322

Toxin (Protein)


Gene name PumA Uniprot ID A0A853H7C5
Locus tag BTK77_RS17610 Protein ID WP_014727945.1
Coordinates 3704168..3704473 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A2S9UB62
Locus tag BTK77_RS17615 Protein ID WP_007852536.1
Coordinates 3704478..3704753 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BTK77_RS17580 (BTK77_017575) 3699841..3700095 + 255 WP_032988097.1 hypothetical protein -
BTK77_RS17585 (BTK77_017580) 3700202..3701794 + 1593 WP_080321953.1 RNA repair transcriptional activator RtcR -
BTK77_RS17590 (BTK77_017585) 3701956..3702891 + 936 WP_004387518.1 ketopantoate/pantoate/pantothenate transporter PanS -
BTK77_RS17595 (BTK77_017590) 3702982..3703254 + 273 WP_007871443.1 DUF3811 domain-containing protein -
BTK77_RS17600 (BTK77_017595) 3703281..3703604 - 324 WP_063265163.1 helix-turn-helix transcriptional regulator -
BTK77_RS17605 (BTK77_017600) 3703601..3703951 - 351 WP_080321952.1 type II toxin-antitoxin system RelE/ParE family toxin -
BTK77_RS17610 (BTK77_017605) 3704168..3704473 + 306 WP_014727945.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BTK77_RS17615 (BTK77_017610) 3704478..3704753 + 276 WP_007852536.1 putative addiction module antidote protein Antitoxin
BTK77_RS17620 (BTK77_017615) 3704770..3706401 - 1632 WP_007891561.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3696562..3704753 8191


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-93)

Antitoxin

Uncharacterized protein HI1420

(3-89)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11588.58 Da        Isoelectric Point: 10.3395

>T209006 WP_014727945.1 NZ_CP078110:3704168-3704473 [Cronobacter sakazakii]
MKHIFQTEVFRRWQASLKDQRVKTMIAARLFRLANGLPGDVKPVGAGISELRIHYGAGYRIYFKQHGEVIVILLCGGDKH
TQDKDILLAKMLADLPENQWR

Download         Length: 306 bp

>T209006 NZ_CP103784:512222-512572 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9597.06 Da        Isoelectric Point: 6.4609

>AT209006 WP_007852536.1 NZ_CP078110:3704478-3704753 [Cronobacter sakazakii]
MKLTEYDPAAALVDDEEIAFFMADALETGDAGHIAKALGVVARAKGMSHIAAQTGLSREQLYRSFSDKGNPTLKTTLAVM
KALGLGLTVKS

Download         Length: 276 bp

>AT209006 NZ_CP103784:511936-512217 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A853H7C5

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9UB62

References