Detailed information of TA system TA209005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 3703281..3703951
• Replicon: chromosome
• Accession: NZ_CP078110
• Organism: Cronobacter sakazakii strain MOD1-H322

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: BTK77_RS17605
• Protein ID: WP_080321952.1
• Coordinates: 3703601..3703951 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A855WTI8
• Locus tag: BTK77_RS17600
• Protein ID: WP_063265163.1
• Coordinates: 3703281..3703604 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTK77_RS17575 (BTK77_017570)	3698593..3699726	-	1134	WP_080321954.1	slipin family protein	-
BTK77_RS17580 (BTK77_017575)	3699841..3700095	+	255	WP_032988097.1	hypothetical protein	-
BTK77_RS17585 (BTK77_017580)	3700202..3701794	+	1593	WP_080321953.1	RNA repair transcriptional activator RtcR	-
BTK77_RS17590 (BTK77_017585)	3701956..3702891	+	936	WP_004387518.1	ketopantoate/pantoate/pantothenate transporter PanS	-
BTK77_RS17595 (BTK77_017590)	3702982..3703254	+	273	WP_007871443.1	DUF3811 domain-containing protein	-
BTK77_RS17600 (BTK77_017595)	3703281..3703604	-	324	WP_063265163.1	helix-turn-helix transcriptional regulator	Antitoxin
BTK77_RS17605 (BTK77_017600)	3703601..3703951	-	351	WP_080321952.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BTK77_RS17610 (BTK77_017605)	3704168..3704473	+	306	WP_014727945.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BTK77_RS17615 (BTK77_017610)	3704478..3704753	+	276	WP_007852536.1	putative addiction module antidote protein	-
BTK77_RS17620 (BTK77_017615)	3704770..3706401	-	1632	WP_007891561.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3696562..3704753	8191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13068.13 Da
• Isoelectric Point: 9.9166

>T209005 WP_080321952.1 NZ_CP078110:c3703951-3703601 [Cronobacter sakazakii]
MIKPLFWVGQARKDLQALPEDVQDLFGYALYLAQQGRRHPQAKPLKGFGGADILEVVEDYQGNAFRAVYTVCLRDAIYIL
HVFQKKSSSGIATPRPEMEKIEQRLKAAQRHAGGLK

Nucleotide

• Length: 351 bp

>T209005 NZ_CP103783:2677590-2677769 [Bacillus subtilis subsp. subtilis str. 168]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11795.87 Da
• Isoelectric Point: 6.3117

>AT209005 WP_063265163.1 NZ_CP078110:c3703604-3703281 [Cronobacter sakazakii]
MTADIERSNGNVYDDLGLIDAEEMQLKAQLAMAISDILKARGLTQQEAARLLGMTQPKLSLLLRGQFRGISETKMMACLM
LLGRDISIVVGEAQQARGKMRLIYEPA

Nucleotide

• Length: 324 bp

>AT209005 NZ_CP103783:c2677915-2677693 [Bacillus subtilis subsp. subtilis str. 168]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Structures

Antitoxin

• AlphaFold DB: A0A855WTI8