Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1694306..1694957 Replicon chromosome
Accession NZ_CP078097
Organism Lacticaseibacillus paracasei subsp. paracasei strain GR0548

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag J5Y16_RS08145 Protein ID WP_003567661.1
Coordinates 1694574..1694957 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag J5Y16_RS08140 Protein ID WP_003567665.1
Coordinates 1694306..1694554 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5Y16_RS08115 (J5Y16_08115) 1689375..1690763 + 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
J5Y16_RS08120 (J5Y16_08120) 1690794..1690937 + 144 WP_003588978.1 hypothetical protein -
J5Y16_RS08125 (J5Y16_08125) 1691063..1692571 + 1509 WP_123018290.1 DEAD/DEAH box helicase -
J5Y16_RS08130 (J5Y16_08130) 1692741..1693115 + 375 WP_003567669.1 holo-ACP synthase -
J5Y16_RS08135 (J5Y16_08135) 1693102..1694238 + 1137 WP_016369534.1 alanine racemase -
J5Y16_RS08140 (J5Y16_08140) 1694306..1694554 + 249 WP_003567665.1 antitoxin Antitoxin
J5Y16_RS08145 (J5Y16_08145) 1694574..1694957 + 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J5Y16_RS08150 (J5Y16_08150) 1695141..1695662 + 522 WP_003567657.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T208988 WP_003567661.1 NZ_CP078097:1694574-1694957 [Lacticaseibacillus paracasei subsp. paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T208988 NZ_CP103771:3632975-3633502 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCATGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATAAGCAAA
GGATTAGGGAGACACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAAGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT208988 WP_003567665.1 NZ_CP078097:1694306-1694554 [Lacticaseibacillus paracasei subsp. paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT208988 NZ_CP103771:3632688-3632978 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References