Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 324365..324936 Replicon chromosome
Accession NZ_CP078094
Organism Enterococcus lactis strain JDM1

Toxin (Protein)


Gene name mazF Uniprot ID R2Q2D5
Locus tag KVG00_RS01560 Protein ID WP_002341043.1
Coordinates 324595..324936 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag KVG00_RS01555 Protein ID WP_025477367.1
Coordinates 324365..324595 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KVG00_RS01535 (KVG00_01535) 320629..321222 + 594 WP_002342793.1 malonate decarboxylase holo-ACP synthase -
KVG00_RS01540 (KVG00_01540) 321246..322331 + 1086 WP_002342794.1 acyltransferase family protein -
KVG00_RS01545 (KVG00_01545) 322353..323222 + 870 WP_002342795.1 triphosphoribosyl-dephospho-CoA synthase MdcB -
KVG00_RS01550 (KVG00_01550) 323334..324098 + 765 WP_002341045.1 TIM44-like domain-containing protein -
KVG00_RS01555 (KVG00_01555) 324365..324595 + 231 WP_025477367.1 hypothetical protein Antitoxin
KVG00_RS01560 (KVG00_01560) 324595..324936 + 342 WP_002341043.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KVG00_RS01565 (KVG00_01565) 325652..327733 + 2082 WP_002342796.1 PTS sugar transporter subunit IIA -
KVG00_RS01570 (KVG00_01570) 327769..328071 + 303 Protein_316 PTS sugar transporter subunit IIB -
KVG00_RS01575 (KVG00_01575) 328097..329386 + 1290 WP_002341041.1 PTS sugar transporter subunit IIC -
KVG00_RS01580 (KVG00_01580) 329405..329812 + 408 Protein_318 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13316.66 Da        Isoelectric Point: 9.9258

>T208981 WP_002341043.1 NZ_CP078094:324595-324936 [Enterococcus lactis]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T208981 NZ_CP103771:504308-504649 [Salmonella enterica]
ATGCAAACCCAATACAACCACCCACACCGGGCGACCCCGTCACAACTATCGCCTGTCGAAATCTGGCAAAAACTGCTGAC
CCATTTACTGGCTAAACATTATGGCCTCGAACTCAGTGACACGCCGTTTAGCGTAGAGAAGGTGATTCAGGAACACATTG
ACGCCGGTATCACGCTGGCTAATGCCGTGAACTTTATTGTAGAGAAATACGAGCTGGTTCGTATCGATCGCAAGGGATTT
AGCTGGCAGGAACAATCACCCTATCTTCGGGCTGTAGACATTCTGCGGGCGCGACAGGCTACGGGTCTGTTGCGACGTCA
ACGCTATCTCGCGGCACACTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT208981 WP_025477367.1 NZ_CP078094:324365-324595 [Enterococcus lactis]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT208981 NZ_CP103771:503923-504264 [Salmonella enterica]
ATGGACACAAATGAAACACAATCCACGCCAGTATGGGGACTACGGAACGATGTCTTCCCGTCATTTGGCGCTCGGTTAGT
TCAGGAAGGTAATCACCTGCACTATCTGGCTGACCGGGCGGGTATTTATGGTGAATTTACCCCGGCGCAACTACAGACGC
TGGACGTCGTATTTCCAATGTTTATTAAACACATGGAGGCCGCTTTACGCACTGGCGCACTCAACCCGCGAGAGGCCAGA
CGCTTTACGACTGAACTCCGGGGCATAACCTGTGAAGCCGATACGTGCGGATCGTTCGGCTACGTTTATCTTTCGCTTTA
CCCAACCATCGTTAAAAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829AD04

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References