Detailed information of TA system TA208977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 185030..186555
• Replicon: chromosome
• Accession: NZ_CP078091
• Organism: Porphyrobacter sp. ULC335

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: KVF90_RS00915
• Protein ID: WP_264392980.1
• Coordinates: 185284..186555 (+)
• Length: 424 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: KVF90_RS00910
• Protein ID: WP_264392979.1
• Coordinates: 185030..185287 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVF90_RS00890 (KVF90_00890)	182395..182700	+	306	WP_264394593.1	conjugal transfer protein TraD	-
KVF90_RS00895 (KVF90_00895)	182723..182944	+	222	WP_264392976.1	conjugal transfer protein TraD	-
KVF90_RS00900 (KVF90_00900)	182991..183932	+	942	WP_264392977.1	HEPN domain-containing protein	-
KVF90_RS00905 (KVF90_00905)	183975..184766	-	792	WP_264392978.1	thermonuclease family protein	-
KVF90_RS00910 (KVF90_00910)	185030..185287	+	258	WP_264392979.1	helix-turn-helix transcriptional regulator	Antitoxin
KVF90_RS00915 (KVF90_00915)	185284..186555	+	1272	WP_264392980.1	type II toxin-antitoxin system HipA family toxin	Toxin
KVF90_RS00920 (KVF90_00920)	186909..187100	-	192	Protein_183	recombinase family protein	-
KVF90_RS00925 (KVF90_00925)	187201..187539	+	339	WP_264392981.1	hypothetical protein	-
KVF90_RS00930 (KVF90_00930)	187674..188963	-	1290	WP_264394595.1	Hsp70 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	179328..217062	37734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (152-380)

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-65)

Sequences

Toxin

Protein

• Length: 424 a.a.
• Molecular weight: 45342.59 Da
• Isoelectric Point: 5.2103

>T208977 WP_264392980.1 NZ_CP078091:185284-186555 [Porphyrobacter sp. ULC335]
MTPPILDTLRVWWDRRQAGELAIDKGGAMHFAYSAEWLADDSAPPLSQALPKRGEAFDDHVCKAVFGGLLPEESQRTAIA
RALGVSPDNPFRLLQALGGDVAGALSFLPAGEEPLQDETNGDAEPLDEGELAALLDRLPAAPMLAGEGGPRLSLAGAQSK
LPVVLTANGRIAVPRPGEPSTHLIKPEPERFAGLAANEAFCLALASAVGLDTARAEWREVDGRPLLLVERYDRVSVGGRT
QRLHQEDFAQALAVPSNRKYAAEGGPTFLDSFALLRSAATRPAREVLKLADAAIFNLIVGNADAHAKNYSLLRLQTGEVV
LAPLYDHVATHMWKELSPSLAMRFGRAATIEELGKDNLERFAEDAGLPAPFVKRRLFQIAVALSREIAKGVFVRGLDDQN
AIAPVAEAVAMRAAYARDGHPST

Nucleotide

• Length: 1272 bp

>T208977 NZ_CP103769:c2643261-2642911 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8790.24 Da
• Isoelectric Point: 4.9814

>AT208977 WP_264392979.1 NZ_CP078091:185030-185287 [Porphyrobacter sp. ULC335]
VLTVTTIGAIIRDERKALGLRQDELAAASGVGLRFLVELERGKPTVQMGKVLDVLAALGCDLQILGPAGDAIVPQPPASV
RSSGE

Nucleotide

• Length: 258 bp

>AT208977 NZ_CP103769:c2643548-2643267 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA