Detailed information of TA system TA208974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2324952..2325927
• Replicon: chromosome
• Accession: NZ_CP078081
• Organism: Bacillus tropicus strain EMB20

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KU891_RS11875
• Protein ID: WP_061403070.1
• Coordinates: 2324952..2325689 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D8GY54
• Locus tag: KU891_RS11880
• Protein ID: WP_000594608.1
• Coordinates: 2325802..2325927 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU891_RS11850 (KU891_11830)	2320302..2321012	+	711	WP_000426882.1	class I SAM-dependent methyltransferase	-
KU891_RS11855 (KU891_11835)	2321132..2321539	+	408	WP_061402803.1	VOC family protein	-
KU891_RS11860 (KU891_11840)	2321585..2323270	-	1686	WP_139889450.1	alpha-keto acid decarboxylase family protein	-
KU891_RS11865 (KU891_11845)	2323377..2323859	+	483	WP_000191902.1	MarR family winged helix-turn-helix transcriptional regulator	-
KU891_RS11870 (KU891_11850)	2324026..2324763	+	738	WP_074563909.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
KU891_RS11875 (KU891_11855)	2324952..2325689	+	738	WP_061403070.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KU891_RS11880 (KU891_11860)	2325802..2325927	+	126	WP_000594608.1	hypothetical protein	Antitoxin
KU891_RS11885 (KU891_11865)	2326003..2326179	+	177	WP_000852620.1	stage II sporulation protein SB	-
KU891_RS11890 (KU891_11870)	2326198..2326587	-	390	WP_000808873.1	YxeA family protein	-
KU891_RS11895 (KU891_11875)	2326803..2328272	+	1470	WP_061402806.1	beta-Ala-His dipeptidase	-
KU891_RS11900 (KU891_11880)	2328818..2329288	-	471	WP_071679620.1	DUF5065 family protein	-
KU891_RS11905 (KU891_11885)	2329743..2330402	+	660	WP_139889451.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28405.13 Da
• Isoelectric Point: 8.2672

>T208974 WP_061403070.1 NZ_CP078081:2324952-2325689 [Bacillus tropicus]
VISNIRIGLFILVIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T208974 NZ_CP103768:c1013705-1013502 [Morganella morganii]
ATGACTAAACTGGACTATCTCATGCGCTTACGTAAGTGCACTTCTATTGAAACACTGGAACGTGTCATTGAGAAGAACAA
ATATGAACTGACTGATGATGAGCTGGAGGTTTTTTATTCAGCAGCCGATCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCATCTGTCTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4878.27 Da
• Isoelectric Point: 3.9507

>AT208974 WP_000594608.1 NZ_CP078081:2325802-2325927 [Bacillus tropicus]
VIKNNDNDFFKEDNEDTTDFSLIKGDTMQQVEDLEDELKRK

Nucleotide

• Length: 126 bp

>AT208974 NZ_CP103768:c1014128-1013769 [Morganella morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACCCTCATCAGAAGCAATAATTATTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGGCCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCGTTTAATGACGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAGGGGCATCTCGTCCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U6H9M7