Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 253926..254568 Replicon chromosome
Accession NZ_CP078081
Organism Bacillus tropicus strain EMB20

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag KU891_RS01415 Protein ID WP_000635963.1
Coordinates 254218..254568 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag KU891_RS01410 Protein ID WP_000004570.1
Coordinates 253926..254213 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU891_RS01385 (KU891_01385) 249244..250206 + 963 WP_061401977.1 UV DNA damage repair endonuclease UvsE -
KU891_RS01390 (KU891_01390) 250199..250771 - 573 WP_071679120.1 rhomboid family intramembrane serine protease -
KU891_RS01395 (KU891_01395) 250864..251223 + 360 WP_000635047.1 holo-ACP synthase -
KU891_RS01400 (KU891_01400) 251380..252330 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
KU891_RS01405 (KU891_01405) 252449..253618 + 1170 WP_000390607.1 alanine racemase -
KU891_RS01410 (KU891_01410) 253926..254213 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
KU891_RS01415 (KU891_01415) 254218..254568 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KU891_RS01420 (KU891_01420) 254636..256804 + 2169 WP_000426229.1 Tex family protein -
KU891_RS01425 (KU891_01425) 256862..256978 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
KU891_RS01430 (KU891_01430) 257174..257632 + 459 WP_000344257.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T208973 WP_000635963.1 NZ_CP078081:254218-254568 [Bacillus tropicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T208973 NZ_CP103765:31594-31941 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAATCTACAGGTTTACGGGCCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCTATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT208973 WP_000004570.1 NZ_CP078081:253926-254213 [Bacillus tropicus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT208973 NZ_CP103765:31941-32240 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATTAGTC
AGCCAGCAGTAACAAAGTTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References