Detailed information of TA system TA208963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 847172..847764
• Replicon: chromosome
• Accession: NZ_CP078069
• Organism: Comamonas sp. NLF-1-9

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KUD94_RS04180
• Protein ID: WP_218239180.1
• Coordinates: 847172..847468 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KUD94_RS04185
• Protein ID: WP_218238562.1
• Coordinates: 847471..847764 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUD94_RS04145 (KUD94_04145)	842343..843908	+	1566	WP_218238557.1	fumarate hydratase	-
KUD94_RS04150 (KUD94_04150)	844178..844681	+	504	WP_218238558.1	DUF421 domain-containing protein	-
KUD94_RS04155 (KUD94_04155)	844840..845286	+	447	WP_218238559.1	hypothetical protein	-
KUD94_RS04160 (KUD94_04160)	845292..845636	+	345	WP_218238560.1	cupin domain-containing protein	-
KUD94_RS04165 (KUD94_04165)	845713..846318	+	606	WP_255569041.1	YdeI/OmpD-associated family protein	-
KUD94_RS04170 (KUD94_04170)	846470..846901	+	432	WP_218238561.1	hypothetical protein	-
KUD94_RS04175 (KUD94_04175)	846983..847087	-	105	Protein_820	putative Fe-S cluster assembly protein SufT	-
KUD94_RS04180 (KUD94_04180)	847172..847468	+	297	WP_218239180.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUD94_RS04185 (KUD94_04185)	847471..847764	+	294	WP_218238562.1	putative addiction module antidote protein	Antitoxin
KUD94_RS04190 (KUD94_04190)	847881..848441	-	561	WP_146913999.1	putative Fe-S cluster assembly protein SufT	-
KUD94_RS04195 (KUD94_04195)	848445..848912	-	468	WP_218238563.1	SUF system NifU family Fe-S cluster assembly protein	-
KUD94_RS04200 (KUD94_04200)	848909..850174	-	1266	WP_255569042.1	aminotransferase class V-fold PLP-dependent enzyme	-
KUD94_RS04205 (KUD94_04205)	850167..851375	-	1209	WP_218238565.1	SufD family Fe-S cluster assembly protein	-
KUD94_RS04210 (KUD94_04210)	851378..852160	-	783	WP_218238566.1	Fe-S cluster assembly ATPase SufC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	745452..855265	109813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11129.81 Da
• Isoelectric Point: 10.2250

>T208963 WP_218239180.1 NZ_CP078069:847172-847468 [Comamonas sp. NLF-1-9]
VYEVLKSSTFDSWLRGLRDRDAKARIEVRIRRLSLGNPGQFRALKEGVNELKIDHGPGYRVYYTFKGKTLILLLCGGDKS
SQGKDIRLAMDIAASWEN

Nucleotide

• Length: 297 bp

>T208963 NZ_CP103762:c4447756-4447466 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10241.59 Da
• Isoelectric Point: 4.7204

>AT208963 WP_218238562.1 NZ_CP078069:847471-847764 [Comamonas sp. NLF-1-9]
MAKAKETFTRYDTADYLETEEDIAAYLTACAEEDDPALFLAALGDVARARNITQLAAQTGLTRMGLYKALSGTGNPSFAT
VSKVAHALGLRITVAAA

Nucleotide

• Length: 294 bp

>AT208963 NZ_CP103762:c4447988-4447746 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA