Detailed information of TA system TA208958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEi(antitoxin)
• Location: 6433852..6434653
• Replicon: chromosome
• Accession: NZ_CP078066
• Organism: Bradyrhizobium arachidis strain Hy4

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: KUL72_RS30240
• Protein ID: WP_258763197.1
• Coordinates: 6433852..6434013 (-)
• Length: 54 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: KUL72_RS30245
• Protein ID: WP_258763199.1
• Coordinates: 6434006..6434653 (-)
• Length: 216 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUL72_RS30220 (KUL72_30070)	6429406..6430935	-	1530	WP_258763193.1	DUF5716 family protein	-
KUL72_RS30225 (KUL72_30075)	6431380..6431631	+	252	WP_080139081.1	helix-turn-helix transcriptional regulator	-
KUL72_RS30230 (KUL72_30080)	6431631..6432950	+	1320	WP_258763195.1	type II toxin-antitoxin system HipA family toxin	-
KUL72_RS30235 (KUL72_30085)	6433424..6433843	-	420	WP_258763196.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
KUL72_RS30240	6433852..6434013	-	162	WP_258763197.1	hypothetical protein	Toxin
KUL72_RS30245 (KUL72_30090)	6434006..6434653	-	648	WP_258763199.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
KUL72_RS30250 (KUL72_30095)	6434789..6435073	+	285	WP_258763200.1	hypothetical protein	-
KUL72_RS30255 (KUL72_30100)	6435123..6435596	+	474	WP_258763201.1	hypothetical protein	-
KUL72_RS30260 (KUL72_30105)	6435872..6436267	+	396	WP_258763202.1	hypothetical protein	-
KUL72_RS30265 (KUL72_30110)	6436340..6436642	+	303	WP_258763203.1	hypothetical protein	-
KUL72_RS30270 (KUL72_30115)	6437135..6437224	+	90	Protein_6005	ATP-binding protein	-
KUL72_RS30275 (KUL72_30120)	6438272..6438796	+	525	WP_258763204.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6308484..6476834	168350

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (13-57)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6193.27 Da
• Isoelectric Point: 10.8676

>T208958 WP_258763197.1 NZ_CP078066:c6434013-6433852 [Bradyrhizobium arachidis]
MRKPPKNVGASVRARLLDLSKERNEPFELILTHYAIERLLYRLSVSQHKTGLF

Nucleotide

• Length: 162 bp

>T208958 NZ_CP103762:3311754-3312140 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 216 a.a.
• Molecular weight: 24360.00 Da
• Isoelectric Point: 10.0422

>AT208958 WP_258763199.1 NZ_CP078066:c6434653-6434006 [Bradyrhizobium arachidis]
MEKEPSRQEDRATSLLRKQGMMRLSEFHEHGITSATISRMEERGLLNQLARGLYQLPDAPLDGNHSLVVAAKLIPNGVVC
YDSALAFHELTDRIPPYVWMGIGQRDWRPKISSPRIEIVRFNQSEFTSGVDQHIIENVSVAIYRPAKTIVDLFKSGKRQK
AFYDSNTGFAHATQAMKKALRLRMTTPAEIARFAVQSNVWEKIVQPRLEALTINA

Nucleotide

• Length: 648 bp

>AT208958 NZ_CP103762:3312130-3312459 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA