208949

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3258391..3258983 Replicon chromosome
Accession NZ_CP078038
Organism Shewanella putrefaciens strain SA70

Toxin (Protein)


Gene name hicA Uniprot ID A0A252ENI2
Locus tag B7D78_RS14240 Protein ID WP_014610888.1
Coordinates 3258391..3258645 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A252ENK1
Locus tag B7D78_RS14245 Protein ID WP_014610929.1
Coordinates 3258642..3258983 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D78_RS23735 3253497..3253898 + 402 WP_256657210.1 zeta toxin family protein -
B7D78_RS23740 3253922..3254224 + 303 WP_256657314.1 zeta toxin family protein -
B7D78_RS14220 (KVP08_014220) 3254735..3255748 - 1014 WP_086904810.1 dTDP-glucose 4,6-dehydratase -
B7D78_RS14225 (KVP08_014225) 3256304..3256686 - 383 Protein_2760 transposase -
B7D78_RS14230 (KVP08_014230) 3256897..3257385 + 489 WP_069453178.1 mobile mystery protein A -
B7D78_RS23745 3257382..3257714 + 333 WP_256657321.1 hypothetical protein -
B7D78_RS23750 3257602..3257991 + 390 WP_256657315.1 mobile mystery protein B -
B7D78_RS14240 (KVP08_014240) 3258391..3258645 + 255 WP_014610888.1 type II toxin-antitoxin system HicA family toxin Toxin
B7D78_RS14245 (KVP08_014245) 3258642..3258983 + 342 WP_014610929.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B7D78_RS14250 (KVP08_014250) 3259147..3259527 - 381 WP_086904820.1 helix-turn-helix transcriptional regulator -
B7D78_RS14255 (KVP08_014255) 3259524..3260891 - 1368 WP_014610930.1 HipA domain-containing protein -
B7D78_RS14260 (KVP08_014260) 3261246..3261545 - 300 Protein_2768 CcdB family protein -
B7D78_RS14265 (KVP08_014265) 3261625..3262029 - 405 WP_069453179.1 IS200/IS605 family transposase -
B7D78_RS14270 (KVP08_014270) 3262076..3263167 + 1092 WP_086904818.1 RNA-guided endonuclease TnpB family protein -
B7D78_RS14275 (KVP08_014275) 3263141..3263689 - 549 WP_217895415.1 N-terminal phage integrase SAM-like domain-containing protein -
B7D78_RS14280 (KVP08_014280) 3263649..3263807 - 159 WP_197721441.1 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3256324..3256764 440


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-75)

Antitoxin

Uncharacterised protein family HicB

(55-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9480.99 Da        Isoelectric Point: 10.1453

>T208949 WP_014610888.1 NZ_CP078038:3258391-3258645 [Shewanella putrefaciens]
MNKKHLRTLTAIFARPVSGAIKWSDIEALFIALGADIEEREGSRIGVVLFGEVQVYHRPHPQKETDKDAVISIKKWLERN
GVKA

Download         Length: 255 bp

>T208949 NZ_CP103762:1100041-1100352 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12379.91 Da        Isoelectric Point: 4.3992

>AT208949 WP_014610929.1 NZ_CP078038:3258642-3258983 [Shewanella putrefaciens]
MKNLMMINGVKAFIDYDPDAETFRGEFVGLNGGADFYGESVAQLEAEGAKSLSVFLEMCQERGIEPYKNFSGKFNVRISP
EVHARLNEIALSQSISLNAAVENAVNDYIAHSA

Download         Length: 342 bp

>AT208949 NZ_CP103762:1100349-1100768 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A252ENI2

Antitoxin

Source ID Structure
AlphaFold DB A0A252ENK1

References