Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 239514..240036 Replicon plasmid pKP_160-2
Accession NZ_CP078034
Organism Klebsiella pneumoniae strain KP_160

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag EGK18_RS27600 Protein ID WP_038991638.1
Coordinates 239752..240036 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag EGK18_RS27595 Protein ID WP_004181777.1
Coordinates 239514..239762 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK18_RS27575 (EGK18_027575) 234723..235544 - 822 WP_004181772.1 hypothetical protein -
EGK18_RS27580 (EGK18_027580) 235606..235959 - 354 WP_004181774.1 hypothetical protein -
EGK18_RS27585 (EGK18_027585) 236104..237090 - 987 WP_040120328.1 hypothetical protein -
EGK18_RS27590 (EGK18_027590) 237424..239223 - 1800 WP_043907032.1 ATP-dependent helicase -
EGK18_RS27595 (EGK18_027595) 239514..239762 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EGK18_RS27600 (EGK18_027600) 239752..240036 + 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGK18_RS27605 (EGK18_027605) 240053..240154 - 102 Protein_272 IS200/IS605 family transposase -
EGK18_RS27610 (EGK18_027610) 240190..241416 + 1227 WP_040120327.1 RNA-guided endonuclease TnpB family protein -
EGK18_RS27615 (EGK18_027615) 241688..241912 - 225 Protein_274 transposase -
EGK18_RS27620 (EGK18_027620) 241991..242419 - 429 WP_077256229.1 IS200/IS605 family transposase -
EGK18_RS27625 (EGK18_027625) 242455..243642 + 1188 WP_043907031.1 RNA-guided endonuclease TnpB family protein -
EGK18_RS29200 243687..244058 - 372 WP_044816264.1 hypothetical protein -
EGK18_RS29205 244055..244399 - 345 WP_014386518.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..319619 319619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T208942 WP_038991638.1 NZ_CP078034:239752-240036 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T208942 NZ_CP103762:216664-216771 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT208942 WP_004181777.1 NZ_CP078034:239514-239762 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT208942 NZ_CP103762:c216615-216552 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References