Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 165561..166312 Replicon plasmid pKP_160-2
Accession NZ_CP078034
Organism Klebsiella pneumoniae strain KP_160

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag EGK18_RS27195 Protein ID WP_014386536.1
Coordinates 165561..166043 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag EGK18_RS27200 Protein ID WP_004902250.1
Coordinates 166034..166312 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK18_RS27175 (EGK18_027175) 161960..162607 - 648 WP_014386537.1 EcsC family protein -
EGK18_RS27180 (EGK18_027180) 162634..163389 - 756 WP_004902235.1 DUF2971 domain-containing protein -
EGK18_RS27185 (EGK18_027185) 163490..163882 - 393 WP_032442757.1 hypothetical protein -
EGK18_RS27190 (EGK18_027190) 163987..164526 - 540 WP_004902239.1 hypothetical protein -
EGK18_RS27195 (EGK18_027195) 165561..166043 - 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
EGK18_RS27200 (EGK18_027200) 166034..166312 - 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
EGK18_RS27205 (EGK18_027205) 166431..166643 - 213 WP_004902255.1 hypothetical protein -
EGK18_RS27210 (EGK18_027210) 166751..167092 - 342 WP_004902257.1 hypothetical protein -
EGK18_RS27215 (EGK18_027215) 167922..168380 - 459 WP_014386535.1 hypothetical protein -
EGK18_RS27220 (EGK18_027220) 169033..169488 - 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
EGK18_RS27225 (EGK18_027225) 169560..169925 + 366 WP_004200999.1 mercuric ion transporter MerT -
EGK18_RS27230 (EGK18_027230) 169941..170216 + 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
EGK18_RS27235 (EGK18_027235) 170244..170669 + 426 WP_000522996.1 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..319619 319619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T208941 WP_014386536.1 NZ_CP078034:c166043-165561 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T208941 NZ_CP103762:216180-216287 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT208941 WP_004902250.1 NZ_CP078034:c166312-166034 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT208941 NZ_CP103762:c216122-216068 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References