Detailed information of TA system TA208940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 89262..90172
• Replicon: plasmid pKP_160-2
• Accession: NZ_CP078034
• Organism: Klebsiella pneumoniae strain KP_160

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: EGK18_RS26815
• Protein ID: WP_004181896.1
• Coordinates: 89702..90172 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: EGK18_RS26810
• Protein ID: WP_004181895.1
• Coordinates: 89262..89705 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGK18_RS26795 (EGK18_026795)	85895..87382	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
EGK18_RS26800 (EGK18_026800)	87468..87890	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
EGK18_RS26805 (EGK18_026805)	87890..89161	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
EGK18_RS26810 (EGK18_026810)	89262..89705	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
EGK18_RS26815 (EGK18_026815)	89702..90172	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
EGK18_RS26820 (EGK18_026820)	90282..90542	-	261	WP_004026352.1	hypothetical protein	-
EGK18_RS26825 (EGK18_026825)	91265..91423	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
EGK18_RS26830 (EGK18_026830)	92026..93000	+	975	WP_043907013.1	hypothetical protein	-
EGK18_RS26835 (EGK18_026835)	93198..93575	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	-	1..319619	319619

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T208940 WP_004181896.1 NZ_CP078034:89702-90172 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T208940 NZ_CP103759:62926-63324 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT208940 WP_004181895.1 NZ_CP078034:89262-89705 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT208940 NZ_CP103759:62699-62926 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9