Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26898..27541 Replicon plasmid pKP_160-2
Accession NZ_CP078034
Organism Klebsiella pneumoniae strain KP_160

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag EGK18_RS26420 Protein ID WP_001044770.1
Coordinates 26898..27314 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag EGK18_RS26425 Protein ID WP_001261282.1
Coordinates 27311..27541 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK18_RS26390 (EGK18_026390) 22066..22854 + 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
EGK18_RS26395 (EGK18_026395) 22914..23738 + 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
EGK18_RS26400 (EGK18_026400) 23938..24255 + 318 Protein_29 recombinase family protein -
EGK18_RS26405 (EGK18_026405) 24438..25298 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
EGK18_RS26415 (EGK18_026415) 26135..26824 - 690 Protein_32 AAA family ATPase -
EGK18_RS26420 (EGK18_026420) 26898..27314 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
EGK18_RS26425 (EGK18_026425) 27311..27541 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EGK18_RS26430 (EGK18_026430) 27498..27959 + 462 WP_072202616.1 hypothetical protein -
EGK18_RS26435 (EGK18_026435) 28103..28453 + 351 WP_000493378.1 hypothetical protein -
EGK18_RS26440 (EGK18_026440) 28504..29247 + 744 WP_000129823.1 hypothetical protein -
EGK18_RS26445 (EGK18_026445) 29244..30020 + 777 WP_000015958.1 site-specific integrase -
EGK18_RS26450 (EGK18_026450) 30078..30335 - 258 WP_000764642.1 hypothetical protein -
EGK18_RS26455 (EGK18_026455) 30464..30568 - 105 WP_032409716.1 hypothetical protein -
EGK18_RS26460 (EGK18_026460) 31103..31969 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 - 1..319619 319619


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T208939 WP_001044770.1 NZ_CP078034:c27314-26898 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T208939 NZ_CP103759:c53970-53821 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT208939 WP_001261282.1 NZ_CP078034:c27541-27311 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT208939 NZ_CP103759:54014-54075 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References