Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5337247..5337872 Replicon chromosome
Accession NZ_CP078033
Organism Klebsiella pneumoniae strain KP_160

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag EGK18_RS25810 Protein ID WP_002882817.1
Coordinates 5337247..5337630 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EGK18_RS25815 Protein ID WP_004150355.1
Coordinates 5337630..5337872 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK18_RS25795 (5334613) 5334613..5335515 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
EGK18_RS25800 (5335512) 5335512..5336147 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
EGK18_RS25805 (5336144) 5336144..5337073 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EGK18_RS25810 (5337247) 5337247..5337630 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
EGK18_RS25815 (5337630) 5337630..5337872 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
EGK18_RS25820 (5338077) 5338077..5338994 + 918 WP_004181612.1 alpha/beta hydrolase -
EGK18_RS25825 (5339008) 5339008..5339949 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
EGK18_RS25830 (5339994) 5339994..5340431 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
EGK18_RS25835 (5340428) 5340428..5341288 - 861 WP_004146232.1 virulence factor BrkB family protein -
EGK18_RS25840 (5341282) 5341282..5341881 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T208938 WP_002882817.1 NZ_CP078033:c5337630-5337247 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T208938 NZ_CP103759:21331-21747 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT208938 WP_004150355.1 NZ_CP078033:c5337872-5337630 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT208938 NZ_CP103759:21104-21334 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References