Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4161497..4162116 Replicon chromosome
Accession NZ_CP078033
Organism Klebsiella pneumoniae strain KP_160

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EGK18_RS20300 Protein ID WP_002892050.1
Coordinates 4161898..4162116 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EGK18_RS20295 Protein ID WP_002892066.1
Coordinates 4161497..4161871 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK18_RS20285 (4156649) 4156649..4157842 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EGK18_RS20290 (4157865) 4157865..4161011 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EGK18_RS20295 (4161497) 4161497..4161871 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EGK18_RS20300 (4161898) 4161898..4162116 + 219 WP_002892050.1 HHA domain-containing protein Toxin
EGK18_RS20305 (4162275) 4162275..4162841 + 567 WP_002892030.1 maltose O-acetyltransferase -
EGK18_RS20310 (4162813) 4162813..4162953 - 141 WP_004147370.1 hypothetical protein -
EGK18_RS20315 (4162974) 4162974..4163444 + 471 WP_002892026.1 YlaC family protein -
EGK18_RS20320 (4163419) 4163419..4164870 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
EGK18_RS20325 (4164971) 4164971..4165669 + 699 WP_004178762.1 GNAT family protein -
EGK18_RS20330 (4165666) 4165666..4165806 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EGK18_RS20335 (4165806) 4165806..4166069 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T208934 WP_002892050.1 NZ_CP078033:4161898-4162116 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T208934 NZ_CP103758:3408895-3409113 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT208934 WP_002892066.1 NZ_CP078033:4161497-4161871 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT208934 NZ_CP103758:3408495-3408869 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References