208921

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 14322..14910 Replicon plasmid pAV_175-4
Accession NZ_CP078030
Organism Acinetobacter variabilis strain AV_175

Toxin (Protein)


Gene name brnT Uniprot ID N8SM64
Locus tag EGK58_RS16695 Protein ID WP_000438825.1
Coordinates 14623..14910 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID D3JXQ4
Locus tag EGK58_RS16690 Protein ID WP_004728120.1
Coordinates 14322..14636 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK58_RS16645 (EGK58_016645) 9493..9813 - 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
EGK58_RS16650 (EGK58_016650) 9953..10177 + 225 WP_125279881.1 SMI1/KNR4 family protein -
EGK58_RS16655 (EGK58_016655) 10199..10582 + 384 WP_001055589.1 transposase -
EGK58_RS16660 (EGK58_016660) 10579..10914 + 336 WP_219014810.1 IS66 family insertion sequence element accessory protein TnpB -
EGK58_RS16665 (EGK58_016665) 10989..12593 + 1605 WP_219014809.1 IS66 family transposase -
EGK58_RS16670 (EGK58_016670) 12651..12890 + 240 WP_267460446.1 SMI1/KNR4 family protein -
EGK58_RS16675 (EGK58_016675) 12970..13251 - 282 WP_000985609.1 putative addiction module antidote protein -
EGK58_RS16680 (EGK58_016680) 13252..13554 - 303 WP_000286964.1 type II toxin-antitoxin system RelE/ParE family toxin -
EGK58_RS16685 (EGK58_016685) 13703..14233 + 531 WP_000172759.1 hypothetical protein -
EGK58_RS16690 (EGK58_016690) 14322..14636 - 315 WP_004728120.1 BrnA antitoxin family protein Antitoxin
EGK58_RS16695 (EGK58_016695) 14623..14910 - 288 WP_000438825.1 BrnT family toxin Toxin
EGK58_RS16700 (EGK58_016700) 15164..16117 - 954 WP_125279689.1 hypothetical protein -
EGK58_RS16705 (EGK58_016705) 16370..17551 + 1182 WP_077448184.1 cation diffusion facilitator family transporter -
EGK58_RS16710 (EGK58_016710) 17833..18399 - 567 WP_125279690.1 plasmid replication DNA-binding protein -
EGK58_RS16715 (EGK58_016715) 18463..19356 - 894 WP_001038666.1 replication initiation protein RepM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(39) / mph(E) / msr(E) - 1..19843 19843
- inside IScluster/Tn tet(39) / mph(E) / msr(E) - 4172..12593 8421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11304.70 Da        Isoelectric Point: 5.6205

>T208921 WP_000438825.1 NZ_CP078030:c14910-14623 [Acinetobacter variabilis]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T208921 NZ_CP103758:185065-185172 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12200.86 Da        Isoelectric Point: 8.4628

>AT208921 WP_004728120.1 NZ_CP078030:c14636-14322 [Acinetobacter variabilis]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT208921 NZ_CP103758:c185008-184951 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8TVQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8TRI9

References